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Pakistan Journal of Biological Sciences
  Year: 2011 | Volume: 14 | Issue: 9 | Page No.: 526-532
DOI: 10.3923/pjbs.2011.526.532
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Immobilised Sarawak Malaysia Yeast Cells for Production of Bioethanol

Masniroszaime Mohd Zain, Noorhisham Tan Kofli and Siti Rozaimah Sheikh Abdullah

Bioethanol production using yeast has become a popular topic due to worrying depleting worldwide fuel reserve. The aim of the study was to investigate the capability of Malaysia yeast strains isolated from starter culture used in traditional fermented food and alcoholic beverages in producing Bioethanol using alginate beads entrapment method. The starter yeast consists of groups of microbes, thus the yeasts were grown in Sabouraud agar to obtain single colony called ST1 (tuak) and ST3 (tapai). The growth in Yeast Potatoes Dextrose (YPD) resulted in specific growth of ST1 at μ = 0.396 h-1 and ST3 at μ = 0.38 h-1, with maximum ethanol production of 7.36 g L-1 observed using ST1 strain. The two strains were then immobilized using calcium alginate entrapment method producing average alginate beads size of 0.51 cm and were grown in different substrates; YPD medium and Local Brown Sugar (LBS) for 8 h in flask. The maximum ethanol concentration measured after 7 h were at 6.63 and 6.59 g L-1 in YPD media and 1.54 and 1.39 g L-1 in LBS media for ST1 and ST3, respectively. The use of LBS as carbon source showed higher yield of product (Yp/s), 0.59 g g-1 compared to YPD, 0.25 g g-1 in ST1 and (Yp/s), 0.54 g g-1 compared to YPD, 0.24 g g-1 in ST3 . This study indicated the possibility of using local strains (ST1 and ST3) to produce bioethanol via immobilization technique with local materials as substrate.
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  •    Investigation of Biogas Production and its Residue with Fertilization Effect from Municipal Waste
  •    A Global Overview of Biomass Potentials for Bioethanol Production: A Renewable Alternative Fuel
  •    Comparison of Sweet Sorghum and Cassava for Ethanol Production by Using Saccharomyces cerevisiae
  •    Bioprocessing of Lignocellulosic Biomass for Production of Bioethanol using Thermotolerant Aspergillus fumigatus under Solid State Fermentation Conditions
  •    Improvement of Bioethanol Production using Amylasic Properties from Bacillus licheniformis and Yeasts Strains Fermentation for Biomass Valorization
How to cite this article:

Masniroszaime Mohd Zain, Noorhisham Tan Kofli and Siti Rozaimah Sheikh Abdullah, 2011. Immobilised Sarawak Malaysia Yeast Cells for Production of Bioethanol. Pakistan Journal of Biological Sciences, 14: 526-532.

DOI: 10.3923/pjbs.2011.526.532






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