Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Pakistan Journal of Biological Sciences
  Year: 2007 | Volume: 10 | Issue: 18 | Page No.: 3072-3078
DOI: 10.3923/pjbs.2007.3072.3078
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

An Analysis on DNA Fingerprints of Thirty Papaya Cultivars (Carica papaya L.), Grown in Thailand with the Use of Amplified Fragment Length Polymorphisms Technique

Janthasri Ratchadaporn , Katengam Sureeporn and U. Khumcha

Abstract:
The experiment was carried out at the Department of Horticulture, Ubon Ratchathani University, Ubon Ratchathani province, Northeast Thailand during June 2002 to May 2003 aims to identify DNA fingerprints of thirty papaya cultivars with the use of Amplified Fragment Length Polymorphisms (AFLP) technique. Papaya cultivars were collected from six different research centers in Thailand. Papaya plants of each cultivar were grown under field conditions up to four months then leaf numbers 2 and 3 of each cultivar (counted from top) were chosen for DNA extraction and the samples were used for AFLP analysis. Out of 64 random primers being used, 55 pairs gave an increase in DNA bands but only 12 pairs of random primers were randomly chosen for the final analysis of the experiment. The results showed that AFLP markers gave Polymorphic Information Contents (PIC) of three ranges i.e., AFLP markers of 235 lied on a PIC range of 0.003-0.05, 47 for a PIC range of 0.15-0.20 and 12 for a PIC range of 0.35-0.40. The results on dendrogram cluster analysis revealed that the thirty papaya cultivars were classified into six groups i.e., (1) Kaeg Dum and Malador (2) Kaeg Nuan (3) Pakchong and Solo (4) Taiwan (5) Co Coa Hai Nan and (6) Sitong. Nevertheless, in spite of the six papaya groups all papaya cultivars were genetically related to each other where diversity among the cultivars was not significantly found.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Emulsion Stability and Vegetable Oil Identification and Adulteration
  •    Emulsion Stability and Vegetable Oil Identification and Adulteration
How to cite this article:

Janthasri Ratchadaporn , Katengam Sureeporn and U. Khumcha , 2007. An Analysis on DNA Fingerprints of Thirty Papaya Cultivars (Carica papaya L.), Grown in Thailand with the Use of Amplified Fragment Length Polymorphisms Technique. Pakistan Journal of Biological Sciences, 10: 3072-3078.

DOI: 10.3923/pjbs.2007.3072.3078

URL: https://scialert.net/abstract/?doi=pjbs.2007.3072.3078

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 

Curve Bottom