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Pakistan Journal of Biological Sciences
  Year: 2002 | Volume: 5 | Issue: 6 | Page No.: 688-692
DOI: 10.3923/pjbs.2002.688.692
Cyclodextrin Glucanotransferase Producing Alkalophilic Bacillus sp. G1: its Cultural Condition and Partial Characterization of the Enzyme
Rosli Md. Illias, Tien Siew Fen , Noor Aini Abdulrashid , Wan Mokhtar Wan Yusoff , Aidil Abd Hamid , Osman Hassan and Kamarulzaman Kamaruddin

Abstract:
A cyclodextrin glucanotransferase producing bacteria was isolated from soil sample and identified as Bacillus sp. G1. Crude CGTase production was observed to be maximum after 28 h incubation at 37o C with CGTase activity reading 19 U/ml. The enzyme production was shown to be growth associated and maximum CGTase production was detected during the decline phase. The effect of nutritional requirements on the CGTase production was carried out in this study. Commercial grade tapioca starch and peptone were found to be the best carbon and nitrogen sources respectively for production of CGTase from Bacillus sp. G1. Effect of mineral salts on the CGTase production showed that MgSO4 gave the highest activity. However nutrient such as urea, CuSO4 and NH4NO3 were found to inhibit the production of CGTase. Characterization of the crude CGTase showed that the optimum temperature and pH for the crude enzyme activity were 70oC and 6.0, respectively.
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How to cite this article:

Rosli Md. Illias, Tien Siew Fen , Noor Aini Abdulrashid , Wan Mokhtar Wan Yusoff , Aidil Abd Hamid , Osman Hassan and Kamarulzaman Kamaruddin , 2002. Cyclodextrin Glucanotransferase Producing Alkalophilic Bacillus sp. G1: its Cultural Condition and Partial Characterization of the Enzyme. Pakistan Journal of Biological Sciences, 5: 688-692.

DOI: 10.3923/pjbs.2002.688.692

URL: https://scialert.net/abstract/?doi=pjbs.2002.688.692

 
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