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Journal of Biological Sciences
  Year: 2010 | Volume: 10 | Issue: 7 | Page No.: 676-681
DOI: 10.3923/jbs.2010.676.681
 
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Production of Cyclodextrin Glycosyltransferase (CGTase) by Bacillus lehensis S8 using Sago Starch as Carbon Source

Pui-Woon Yap, Arbakariya B. Ariff, Kwan-Kit Woo and Siew-Ling Hii

Abstract:
Production of cyclodextrin glycosyltransferase (CGTase) is influenced by the reaction of the CGTase-producing strain towards various types of substrates. Variations in environmental factors such as concentrations of carbon and nitrogen sources possess significant effects on CGTase production. The present study was conducted with the prime purpose to optimise the cultivation medium in enhancing the CGTase production by a locally isolated alkalophilic Bacillus sp. The CGTase fermentation processes were performed in 250 mL Erlenmeyer flasks containing 200 mL of production medium with continuous shaking at 200 rpm and 37°C. Optimisation process was conducted by using change-a-factor-at-a-time method. From the study, an indigenous Malaysian carbon source, i.e., sago starch was found capable in improving the CGTase production with the CGTase yield of 18452 U g-1 at 0.1% w/v of starch. In addition to that, by using yeast extract as the sole nitrogen source in the medium, the CGTase excretion by the isolate is greatly enhanced as compared to the basal medium which employed two types of nitrogenous compounds. The optimised growth medium that has been successfully developed for high level of CGTase production by using the locally isolated Bacillus lehensis in 250 mL Erlenmeyer flask is comprised of (% w/v): 0.1% sago starch, 1% yeast extract, 1% sodium carbonate, 0.009% magnesium sulphate and 0.1% di-potassium hydrogen phosphate.
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How to cite this article:

Pui-Woon Yap, Arbakariya B. Ariff, Kwan-Kit Woo and Siew-Ling Hii, 2010. Production of Cyclodextrin Glycosyltransferase (CGTase) by Bacillus lehensis S8 using Sago Starch as Carbon Source. Journal of Biological Sciences, 10: 676-681.

DOI: 10.3923/jbs.2010.676.681

URL: https://scialert.net/abstract/?doi=jbs.2010.676.681

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