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Pakistan Journal of Biological Sciences
  Year: 2000 | Volume: 3 | Issue: 1 | Page No.: 35-37
DOI: 10.3923/pjbs.2000.35.37
 
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Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli O157:H7

Ooi Wai Ling , Son Radu , Gulam Rusul , Mohamed Ismail Abdul Karim , Endang Purwati and Samuel Lihan

Abstract:
A total of 30 strains of Escherichia coli O157:H7 isolated from beef and chicken burger were characterized by Enterobacterial Repetitive Intragenic Consensus (ERIC) genotyping. The ERIC polymorphism patterns obtained as illustrated in a dendrogram showed a significant discriminatory fingerprint among the 30 E. coli O157:H7 strains. Nearly every isolates had a unique fingerprint and that there were no bands that were highly conserved among the isolates. This study suggests that there is considerable genetic heterogeneity among the E. coli O157:H7 strains by ERIC PCR, and that this has application in screening strains from clinical or food samples to detect a virulent strain with a known fingerprint, and to trace its dissemination.
Citation
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How to cite this article:

Ooi Wai Ling , Son Radu , Gulam Rusul , Mohamed Ismail Abdul Karim , Endang Purwati and Samuel Lihan , 2000. Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli O157:H7. Pakistan Journal of Biological Sciences, 3: 35-37.

DOI: 10.3923/pjbs.2000.35.37

URL: https://scialert.net/abstract/?doi=pjbs.2000.35.37

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