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Research Journal of Parasitology
  Year: 2011 | Volume: 6 | Issue: 3 | Page No.: 90-100
DOI: 10.3923/jp.2011.90.100
 
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Efficacy of Sandwich and Dot-ELISA in Diagnosis of Fascioliasis using a Pair of Polyclonal Antibodies Against Cathepsin L Antigen in Naturally Infected Sheep
Tarek M. Diab, Ibrahim Rabia, Wafaa E.L. Komy and Azza E.L. Amir

Abstract:
The present study was designed to prepare a pair of polyclonal antibodies (PAbs) (IgG4 A7 and IgG4 B12) against Fasciola gigantica Cathepsin L. (CL) and used them in development of sandwich ELISA and dot-ELISA to detect Circulating Fasciola Antigens (CFA) in serum samples collected from a total of 157 sheep. The first PAb was used as antigen capturing Ab and the second as horse reddish peroxidase-conjugated antigen detecting Ab. After slaughtering, gross inspection of liver and parasitological stool examination, sheep were divided into Fasciola positive group (97 sheep), other helminthic infection group (30 sheep) and healthy control group (30 sheep). Fasciola antigens detected in serum of sheep by ELISA showed 94.8% sensitivity and 95% specificity. Dot-ELISA sensitivity was found to be 98.9% and specificity was 98.3%. In conclusion, Dot-ELISA gives better sensitivity and specificity than sandwich ELISA and easy to perform method for the rapid diagnosis of fascioliasis.
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How to cite this article:

Tarek M. Diab, Ibrahim Rabia, Wafaa E.L. Komy and Azza E.L. Amir, 2011. Efficacy of Sandwich and Dot-ELISA in Diagnosis of Fascioliasis using a Pair of Polyclonal Antibodies Against Cathepsin L Antigen in Naturally Infected Sheep. Research Journal of Parasitology, 6: 90-100.

DOI: 10.3923/jp.2011.90.100

URL: https://scialert.net/abstract/?doi=jp.2011.90.100

 
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