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Research Journal of Parasitology

Year: 2009 | Volume: 4 | Issue: 3 | Page No.: 70-78
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Authors

E. Miranda-Miranda

R. Cossio-Bayugar

M.D.R. Quezada-Delgado

F. Olvera-Valencia

S. Neri-Orantes

Keywords

An acaricide-resistant and an acaricide-susceptible strain of Rhipicephalus microplus ticks, were evaluated during a 40 day larval development period for carboxylesterase expression on SDS-PAGE, with the objective of establishing the optimal larval age for carboxylesterase specific activity measurement. Experimental results demonstrated that carboxylesterase expression is located at 62 and 55 kDa polypeptides that exhibited enzymatic specific activity reaching a maximum between day 20 and 30 of larval development, larvae of this age showed carboxylesterase activity statistically higher (p<0.001 unpaired t-test) in acaricide resistant ticks when compared both strains, additionally, carboxylesterase activity in both strains was found to coincide with the vitellin degradation process. The assessment of the results suggested that carboxylesterase active enzymes are mature polypeptide products originating from higher mass protein precursors associated with the yolk nutrient storage reserves in preparation to pre-starving conditions. Experimental data obtained during this study demonstrated that in order to obtain reproducible results of carboxylesterase expression levels associated to acaricide resistance by zymograms, the enzymatic assays should be performed on 20 to 30 day old larvae.
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How to cite this article

E. Miranda-Miranda, R. Cossio-Bayugar, M.D.R. Quezada-Delgado, F. Olvera-Valencia and S. Neri-Orantes, 2009. Age-Induced Carboxylesterase Expression in Acaricide-Resistant Rhipicephalus microplus. Research Journal of Parasitology, 4: 70-78.

URL: https://scialert.net/abstract/?doi=jp.2009.70.78

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