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Journal of Medical Sciences

Year: 2006 | Volume: 6 | Issue: 1 | Page No.: 79-86
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Authors

Mehmet Ozaslan

Canan Can

Arzu Barsgan

Keywords

Research Article

Mutations on S Gene Region of Hepatitis B Virus Genotype D and Biological Significant Analyses of These Mutations

Mehmet Ozaslan, Canan Can and Arzu Barsgan
S gene region of HBV-DNA is responsible for expression of surface antigens and includes a-determinant region. Thus, mutation(s) in this region would afford HBV variants a distinct survival advantage. The aim of this study was to search the mutations of the S gene region in different patient groups infected with Genotype D variants and was to analyse biological significance of these mutations. Beside this, we investigated S gene mutation inductance among family members. Forty HBV-DNA positive patients were determined among 132 HBsAg carriers by first stage of Semi-Nested PCR. Genotypes and subtypes were established by sequencing of the amplified S gene regions. Variants were compared with original sequences of these serotypes and mutations were fixed. All variants were designated as Genotype D and subtype ayw3. Ten kinds of point mutations were identified within the S region. The rates of mutations were determined in chronic patients and family members as being maximum among the other groups. The amino acid mutations of 125 (M→T) and 127 (T→P) were found on the first loop of a-determinant. The other consequence was mutation inductance in a family member. We found some mutations on S gene region known to be stable and observed that some of these mutations were effective on S gene expressions. Identified mutations on a-determinant may provide advantage to mutant about escaping from immune system.
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