Journal of Medical Sciences1682-44741812-5727Asian Network for Scientific Information10.3923/jms.2006.79.86OzaslanMehmet CanCanan BarsganArzu 1200661S gene region of HBV-DNA is responsible for expression of surface antigens
and includes a-determinant region. Thus, mutation(s) in this region would afford
HBV variants a distinct survival advantage. The aim of this study was to search
the mutations of the S gene region in different patient groups infected with
Genotype D variants and was to analyse biological significance of these mutations.
Beside this, we investigated S gene mutation inductance among family members.
Forty HBV-DNA positive patients were determined among 132 HBsAg carriers by
first stage of Semi-Nested PCR. Genotypes and subtypes were established by sequencing
of the amplified S gene regions. Variants were compared with original sequences
of these serotypes and mutations were fixed. All variants were designated as
Genotype D and subtype ayw3. Ten kinds of point mutations were identified within
the S region. The rates of mutations were determined in chronic patients and
family members as being maximum among the other groups. The amino acid mutations
of 125 (M→T) and 127 (T→P) were found on the first loop of a-determinant.
The other consequence was mutation inductance in a family member. We found some
mutations on S gene region known to be stable and observed that some of these
mutations were effective on S gene expressions. Identified mutations on a-determinant
may provide advantage to mutant about escaping from immune system.]]>Kao, J.H.,200217643643Aydin, F.,2002Hunt, C.M., J.M. McGill and M.I. Allen,20023110371044Erol, S., O. Zulal, E. Mustafa and A. Mehmet,200315345349Carman, W.F.,199741120Yamamoto, K., M. Horikita and F. Tsuda,19946826712676Le Bouvier, G.L., R.W. McCollum, W.J. Hierholzer, G.R. Irwin, S. Krugman and J.P. Giles,1972222928930Courouce-Pauty, A.M., J.M. Lemaire and J.F. Roux,197835304308Norder, H., A.M. Courouce, P. Coursaget, J.M. Echevarria and S.D. Lee et al.,200447289309Ogata, N., P.J. Cote, A.R. Zanetti, R.H. Miller and M. Shapiro et al.,199930779786Ogata, N., A.R. Zanetti, M. Yu, R.H. Miller and R.H. Purcell,1997175511523Carman, W.F., J. Korula and L. Wallace,199534514061407Kidd-Ljunggren, K., Y. Miyakawa and A.H. Kidd,20028312671280Bozdayi, A.M., H. Bozkaya, A.R. Turkyilmaz, M. Saryodlu and H. Cetinkaya et al.,20012191101Guldas, N.S. and Y.H. Abacioglu,200432344349Bozdayi, A.M., H. Bozkaya, A. Turkyilmaz, N. Aslan and H. Verdi et al.,199927357360Xinghua, H., L. Dongdong, J. Guoqing, S. Yan and M. Lijie et al.,2004996368Carman, W.F., A.R. Zanetti, P. Karayiannis, J. Waters and G. Manzillo et al.,1990336325329Tindall, K.R. and T.A. Kunkel,19882760086013Wallace, L.A. and W.F. Carman,1994248085Fiordalisi, G., F. Ghiotto, F. Castelnuovo, D. Primi and E. Cariani,199420487493Brown, S.E., C.R. Howard, A.J. Zuckerman and M.W. Steward,19842184187Yamamoto, K., M. Horikita, F. Tsuda, K. Itoh and Y.S. Akahane et al.,19953884887Ustacelebi, O.,19991999