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Research Journal of Microbiology
  Year: 2012 | Volume: 7 | Issue: 7 | Page No.: 327-336
DOI: 10.3923/jm.2012.327.336
Screening and Optimal Protease Production by Bacillus sp. Sw-2 Using Low Cost Substrate Medium
U.O. George-Okafor and E.E. Mike-Anosike

Abstract:
The production of most bacterial proteases has always been with expensive refined substrates which tend to increase the production cost. Hence, a low-cost substrates and culture conditions capable of optimizing protease production by an isolated Bacillus sp. were studied. Bacillus sp. SW-2 which demonstrated the highest protease activity was selected and used for protease production by shake-flask fermentation technique at 180 rpm. The maximum protease yield for 72 h (2.697±0.19 IU mL-1) was achieved under optimized culture conditions of pH 9.0, temperature of 45°C and 5% inoculums density with soy meal (1%) and sugar cane bagasse (1%) as nitrogen and carbon sources of the fermentation medium. The protease yield obtained from using the cheap substrates at 72 h incubation was significantly (p>0.05) higher than that obtained from expensive substrates. The protease achieved >85.7±0.08% hydrolytic activities on the tested nitrogen wastes with soybean waste being the mostly hydrolyzed (96.3±0.13%). This result indicated the use of soy meal and sugar cane bagasse as rich substrates for maximum protease yield and the enzyme hydrolytic activity on nitrogen wastes suggests its application in environmental waste degradation.
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How to cite this article:

U.O. George-Okafor and E.E. Mike-Anosike, 2012. Screening and Optimal Protease Production by Bacillus sp. Sw-2 Using Low Cost Substrate Medium. Research Journal of Microbiology, 7: 327-336.

DOI: 10.3923/jm.2012.327.336

URL: https://scialert.net/abstract/?doi=jm.2012.327.336

 
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