Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Research Journal of Microbiology
  Year: 2011 | Volume: 6 | Issue: 2 | Page No.: 132-139
DOI: 10.3923/jm.2011.132.139
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Detection of Escherichia coli O157:H7 in Wildlife from Disturbed Habitats in Sarawak, Malaysia
K. Apun, K.L. Kho, Y.L. Chong, F.H. Hashimatul, M.T. Abdullah, M.A. Rahman, M.B. Lesley and L. Samuel

Abstract:
This study was carried out to assess the occurrence of Escherichia coli and to detect the pathogenic strain Escherichia coli O157:H7 in birds, bats and rodents from disturbed habitats comprising of two urban forests and an oil palm plantation habitats located along the Rejang Basin, Sibu in the state of Sarawak, using both standard microbiological and molecular techniques. A total of 105 bird hosts, 44 of rodent hosts and 84 bat hosts represented 48 species of birds, one species of rodent and ten species of bats were screened for the presence of Escherichia coli. The representative isolates were cultured on a highly selective agar, Cefaxime-tellurite sorbitol MacConkey agar for the detection of Escherichia coli O157:H7. From the microbiological analysis, the overall occurrences of Escherichia coli in the hosts were 43% in rodents, 18% in birds and 11% in bats. The isolates were tested for the presence of pathogenic Escherichia coli O157:H7 strain by multiplex PCR method targeting the slt-I, slt-II, rfbE and fliCH7 genes. The slt-I, slt-II, rfbE genes were not detected in any of the E. coli isolates. This study indicated that bats, birds, or rodents from these habitats in Sarawak did not serve as an important reservoir of Escherichia coli O157:H7 and thus were of no risk in the epidemiologic cycle of emerging enteric bacterial zoonoses in the state of Sarawak, Malaysia.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Incidence of Calf Diarrhea in Cattle and Buffalo Calves in Uttar Pradesh, India
  •    Isolation of Escherichia coli from Ducks and Duck Related Samples
  •    Escherichia Coli, it Prevalence and Antibiotic Resistant in Malaysia: A Mini Review
How to cite this article:

K. Apun, K.L. Kho, Y.L. Chong, F.H. Hashimatul, M.T. Abdullah, M.A. Rahman, M.B. Lesley and L. Samuel, 2011. Detection of Escherichia coli O157:H7 in Wildlife from Disturbed Habitats in Sarawak, Malaysia. Research Journal of Microbiology, 6: 132-139.

DOI: 10.3923/jm.2011.132.139

URL: https://scialert.net/abstract/?doi=jm.2011.132.139

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

       

       

Curve Bottom