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Journal of Fisheries and Aquatic Science

Year: 2013 | Volume: 8 | Issue: 4 | Page No.: 494-505
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Authors

Herpandi

N. Huda

A. Rosma

W.A. Wan Nadiah

Keywords

Research Article

Optimizing the Enzymatic Hydrolysis of Skipjack Tuna (Katsuwonus pelamis) Dark Flesh Using Alcalase® Enzyme: A Response Surface Approach

Herpandi, N. Huda, A. Rosma and W.A. Wan Nadiah
The goal of this study was to optimize enzymatic hydrolysis of the dark flesh of skipjack tuna (Katsuwonus pelamis). Alcalase 2.4 L® was used as the hydrolytic enzyme at various concentrations (1, 1.5, 2, 2.5 and 3% w/w) at pHs from 6 to 10, temperatures from 35 to 75°C and times of 2 to 6 h. The experiment was performed following a five-level second-order central composite design with six replications at the center points. The high coefficients of determination for degree of hydrolysis (R2 = 0.8909) and free tryptophan content (R2 = 0.9732) indicate the suitability of the design for predicting the responses. The optimum hydrolytic conditions predicted by the response surface methodology were 2% Alcalase® at pH 8.86 and 65.4°C for 5.74 h. These conditions resulted in an experimental degree of hydrolysis of 20.74% and free tryptophan content of 102.23 mg kg-1.
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