
	Asian Journal of Biochemistry
	Year: 2011 \| Volume: 6 \| Issue: 4 \| Page No.: 357-365 DOI: 10.3923/ajb.2011.357.365

Application of Response Surface Methodology for the Determination of Optimum Reaction Conditions (Temperature and pH) for Starch Hydrolysis by α-Amylase

Nivedita Jaiswal, Om Prakash, Mahe Talat, S.H. Hasan and Rajesh Kumar Pandey

Abstract: α-amylase from soybean seeds was purified to apparent homogeneity by affinity precipitation via entrapment in alginate with 84% recovery and about 20-fold purification. The α-amylase activity and stability was characterized at various pH and temperature. Response Surface Methodology (RSM) using two-level-two-factor full factorial Central Composite Design (CCD) model was employed to optimize process parameters like pH and temperature which affects the kinetics of α-amylase catalyzed hydrolysis of starch. The results predicted by the design were found in good agreement (R² = 97.85%) with the experimental results indicating the applicability of proposed model. The multiple regression Analysis and ANOVA showed the individual and cumulative effect of pH and temperature on enzyme activity indicating that the activity increased with the increase of pH unto 5.5 and temperature 75°C. Thus, the RSM was successful in determining the optimum reaction conditions for starch hydrolysis by α-amylase.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Optimizing the Enzymatic Hydrolysis of Skipjack Tuna (Katsuwonus pelamis) Dark Flesh Using Alcalase^® Enzyme: A Response Surface Approach

•	Influence of Stirrer Speed on the Morphology of Aspergillus carbonarius var (Bainier) Thom IMI 366159 During Raw Starch Digesting Amylase Production

•	Screening and Optimization of Medium Composition for Mannanase Production by Aspergillus terreus SUK-1 in Solid State Fermentation Using Statistical Experimental Methods

•	Statistical Assessment of Medium Components by Factorial Design and Surface Methodology of L-Asparaginase Production by Isolated Streptomyces radiopugnans MS1 in Submerged Fermentation using Tapioca Effluent

•	Extraction of ß-glucan from Oat and its Interaction with Glucose and Lipoprotein Profile

•	Optimization of Cyclodextrin Glycosyltransferase Production by Response Surface Methodology Approach

•	Optimization of Ultrasonic Extraction of Polysaccharides from Chinese Malted Sorghum Using Response Surface Methodology

•	Response Surface Method as an Efficient Tool for Medium Optimisation

How to cite this article:

Nivedita Jaiswal, Om Prakash, Mahe Talat, S.H. Hasan and Rajesh Kumar Pandey, 2011. Application of Response Surface Methodology for the Determination of Optimum Reaction Conditions (Temperature and pH) for Starch Hydrolysis by α-Amylase. Asian Journal of Biochemistry, 6: 357-365.

DOI: 10.3923/ajb.2011.357.365

URL: https://scialert.net/abstract/?doi=ajb.2011.357.365

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		FSTA
		Google Scholar

Science Alert