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Journal of Biological Sciences

Year: 2020 | Volume: 20 | Issue: 2 | Page No.: 56-64
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Authors

F.N. Okoh

F.N. Okoh

O.O. Odikamnoro

O.O. Odikamnoro

K.N. Ovia

B.O. Eromonsele

S.E. Ibenye

Keywords

Research Article

Detection of Asymptomatic Malaria Parasites by Microscopy and CareStartTM Malaria HRP2/pLDH(Pf) among Newly Admitted Students on a University Campus, Southeast Nigeria

F.N. Okoh F.N. Okoh's LiveDNA, O.O. Odikamnoro O.O. Odikamnoro's LiveDNA, K.N. Ovia, B.O. Eromonsele and S.E. Ibenye
Background and Objective: Different types of malaria parasites affect human red blood cells. It exists in tropical regions and is transmitted by female Anopheles mosquito. Healthy subjects recorded wide prevalence of asymptomatic malaria parasites wherever malaria disease is endemic. This study determined the occurrence of asymptomatic malaria parasites among the newly admitted students on a University Campus, Southeast Nigeria. Materials and Methods: Blood samples of 210 students were investigated using microscopy and CareStartTM malaria HRP2/pLDH(Pf ) rapid diagnostic test. Dipstick antigen-capture assay was done according to manufacturer’s instructions. Blood smears were prepared and examined following standard procedure. Data were analyzed using Statistical Package for the Social Sciences and Chi-square. Accuracy of CareStartTM Malaria HRP2/pLDH(Pf ) were also determined. Results: Forty-six (21.9%) students were identified as being infected with malaria parasites by microscopy. CareStartTM Malaria HRP2/pLDH(Pf ) showed that out of 210 students, 40 (19.0%) were infected. Only Plasmodium falciparum was identified. The accuracy, sensitivity, specificity, positive and negative predictive values of CareStartTM Malaria HRP2/pLDH(Pf ) were 97.1, 87, 100, 100 and 96.5%,respectively, well correlated with microscopy. The Likelihood ratio for positive and negative results were -87.9 and -86%, respectively. Both diagnostic tools showed that ages 24-27 years’ age range had the highest (27.3%) percentage parasite positive respectively (p>0.05) and female gender recorded higher percentage parasite positive with both test tools (p>0.05). Conclusion: CareStartTM Malaria HRP2/pLDH(Pf ) can reliably be used for malaria diagnosis in areas where microscopy is not easily accessible. Malaria prevention activities should be put in place by the University management.
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How to cite this article

F.N. Okoh, O.O. Odikamnoro, K.N. Ovia, B.O. Eromonsele and S.E. Ibenye, 2020. Detection of Asymptomatic Malaria Parasites by Microscopy and CareStartTM Malaria HRP2/pLDH(Pf) among Newly Admitted Students on a University Campus, Southeast Nigeria. Journal of Biological Sciences, 20: 56-64.

DOI: 10.3923/jbs.2020.56.64

URL: https://scialert.net/abstract/?doi=jbs.2020.56.64

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