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Journal of Biological Sciences

Year: 2014 | Volume: 14 | Issue: 7 | Page No.: 480-484
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Authors

Monir Doudi

Mahbubeh Setorki

Keywords

Research Article

Effect of Nanoparticles Fe4NiO4Zn on Liver Enzymes-White Blood Cell and Hematocrit in Wistar Rat

Monir Doudi and Mahbubeh Setorki
Pathogenic mechanisms initiated by nanoparticles, has been demonstrated by the effects of inflammation such as fibrosis-oxidative stress and DNA damage. Aim of this study is, to determine the effects of nanoparticles Fe4NiO4Zn on the number of leukocytes, HCT, platelet counts and liver enzymes in rats. Twenty four male Wistar rat weighing 234±43 g were used in the experiments. Animals were randomly divided into groups, two Fe4NiO4Zn nanoparticle-treated rat groups (1, 2) and one control group. Group 1 and 2 received 5 cc of solution containing 100, 200 ppm Fe4NiO4Zn via IP injection for 7 successive days. The control group was treated with 5 cc normal saline with same procedure. Then, several biochemical parameters such as number of leukocytes (neutrophil-lymphocyte), HCT (hematocrit), platelet counts, Serum Glutamate Oxaloacetat Transaminase (SGOT) and Serum Glutamate Pyrvate Transaminase (SGPT) were evaluated at various time intervals (1, 2, 7 and 14 days). Average SGOT, 14 days after the intervention, in the control group with group 1 (p<0.0001) and group 2 (p = 0.007), average SGPT, 2 days after the intervention, in the control group with group 1 (p = 0.040) and group 2 (p = 0.036) and 7 days after intervention, in the control group with group 1 (p<0.0001) and group 2 (p<0.0001) indicates statistically significant difference (reduction). In the second phase, a significant difference between the control group and group 1 (p = 0.033) and group 2 (p = 0.002) was observed in conjunction with platelet factor. Average WBC, 2 days after the intervention in control group with group 1 (p = 0.046) and 14 days after intervention in the control group with group 2 (p = 0.004) showed statistically significant differences (increasing). Neutrophil average of 14 days in control group with group 1 p = 0.006) and group 2 (p<0.0001 indicates a significant difference (increasing). In four cases, the mean hematocrite and lymphocyte factors among three groups were identical (p>0.05). The Fe2NiO4 nanoparticles stimulate the immune system and inflammatory responses and cause reduction of liver enzymes (in vivo condition). Perhaps the most important reason for the lack of toxic effects of nanoparticles are rapidly removed from the circulation by the reticuloendothelial system in the liver-spleen and lymph nodes. Of course, the rapid elimination occurs after the phenomenon of opsonisation (accumulation of blood proteins in the particles) that causes stimulate the immune system and disposal of nanoparticles.
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