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Journal of Biological Sciences

Year: 2013 | Volume: 13 | Issue: 1 | Page No.: 33-37
Research Article

Method Development and Validation of Nitrofuran Metabolites in Shrimp by Liquid Chromatographic Mass Spectrometric System

M.B. Hossain
Department of Fisheries, Matshya Bhaban, Ramna, Dhaka, 1000, Bangladesh
S. Ahmed
Department of Fisheries, Matshya Bhaban, Ramna, Dhaka, 1000, Bangladesh
M.F. Rahman
Department of Fisheries, Matshya Bhaban, Ramna, Dhaka, 1000, Bangladesh
B.Y. Kamaruzzaman
Institute of Oceanography and Maritime Studies (INOCEM), Kulliyyah of Science, International Islamic University Malaysia, Jalan Istana Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia
K.C.A. Jalal
Institute of Oceanography and Maritime Studies (INOCEM), Kulliyyah of Science, International Islamic University Malaysia, Jalan Istana Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia
S.M.N. Amin
Department of Aquaculture, Faculty of Agriculture, 43400 Serdang, Selangor, Malaysia
Liquid Chromatographic Mass Spectrometric System (LCMSMS) method was developed and validated in shrimp matrix for determination of nitrofuran metabolites in the laboratory of fish inspection and quality control, Department of Fisheries, Matshya Bhaban, Dhaka, Bangladesh. This confirmatory method was used for testing samples for residues of the metabolites of the following nitrofuran compounds, furazolidone (3-amino-2-oxazolidinone, AOZ); furaltadone (5-methyl-morpholino-3-amino-2-oxazolidinone, AMOZ); nitrofurazone (semicarbazide, SEM) and nitrofurantoin (1-aminohydantoin, AHD) in shrimp. The data were generated (3 levels and seven replicates per level) on each of three days for shrimp. The mean recoveries from the tissues were 88-110%, the decision limits (CCα) were 0.12-0.23 ppb and the detection capabilities (CCα) 0.21-0.38 ppb. CCα and CCβ were calculated using the procedure set out in ISO Guide 11843. Nevertheless, CCα value for all nitrofuran metabolites can be investigated and meet the specified easily met the specified EU MRPL of 1 μg kg-1 ranged from 0.12-0.23 μg kg-1 and suitable for routine quality control operations.
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How to cite this article

M.B. Hossain, S. Ahmed, M.F. Rahman, B.Y. Kamaruzzaman, K.C.A. Jalal and S.M.N. Amin, 2013. Method Development and Validation of Nitrofuran Metabolites in Shrimp by Liquid Chromatographic Mass Spectrometric System. Journal of Biological Sciences, 13: 33-37.

DOI: 10.3923/jbs.2013.33.37

URL: https://scialert.net/abstract/?doi=jbs.2013.33.37

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