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Journal of Biological Sciences

Year: 2008 | Volume: 8 | Issue: 6 | Page No.: 1110-1114
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Authors

S.K. Mondal

U.K. Mazumder

N.B. Mondal

S. Banerjee

Keywords

Research Article

Optimization of Rat Liver Microsomal Stability Assay Using HPLC

S.K. Mondal, U.K. Mazumder, N.B. Mondal and S. Banerjee
Microsomal stability of atenolol, propranolol HCl, verapamil HCl, imipramine HCl, midazolam HCl andrographolide and 14-Deoxyandrographolide was performed and quantitation was done by HPLC. The results with a single batch microsome showed good day-to-day reproducibility and variation in percent stability was within ± 5.71% of the average for all the compounds for the entire study period of 2 years. Microsome was found to be stable at -80 °C store for at least upto 2 years. Two batches of microsomes from each of 3 vendors were also used for stability study where similar type of animal, microsome preparation method and assay protocol were used. The findings demonstrated variations for both batch to batch and vendor to vendor were within 10%. Results indicated that variability in stability data can be minimized by selecting a fixed type of animal, keeping similar method of microsome preparation and following same assay protocol.
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Comments

p.giriraj Reply

dear sir,
its a very good study,i would like to learn more from this study.i also like to do my project work in the same study. so please send me contact information to the crosponding author and more deatials regarding this
thank you
p.giriraj

Susanta Kumar ondal Reply

Dear Giriraj,
Thanks for your comments. I am glad that the paper is useful to you. you may contact me for futher information.
Best wishes,
Susanta

Susanta Kumar Mondal Reply

Thanks for your comments

Best regards
Susanta

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