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Journal of Biological Sciences
  Year: 2008 | Volume: 8 | Issue: 6 | Page No.: 1110-1114
DOI: 10.3923/jbs.2008.1110.1114
Optimization of Rat Liver Microsomal Stability Assay Using HPLC
S.K. Mondal, U.K. Mazumder, N.B. Mondal and S. Banerjee

Abstract:
Microsomal stability of atenolol, propranolol HCl, verapamil HCl, imipramine HCl, midazolam HCl andrographolide and 14-Deoxyandrographolide was performed and quantitation was done by HPLC. The results with a single batch microsome showed good day-to-day reproducibility and variation in percent stability was within ± 5.71% of the average for all the compounds for the entire study period of 2 years. Microsome was found to be stable at -80 °C store for at least upto 2 years. Two batches of microsomes from each of 3 vendors were also used for stability study where similar type of animal, microsome preparation method and assay protocol were used. The findings demonstrated variations for both batch to batch and vendor to vendor were within 10%. Results indicated that variability in stability data can be minimized by selecting a fixed type of animal, keeping similar method of microsome preparation and following same assay protocol.
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How to cite this article:

S.K. Mondal, U.K. Mazumder, N.B. Mondal and S. Banerjee, 2008. Optimization of Rat Liver Microsomal Stability Assay Using HPLC. Journal of Biological Sciences, 8: 1110-1114.

DOI: 10.3923/jbs.2008.1110.1114

URL: https://scialert.net/abstract/?doi=jbs.2008.1110.1114

COMMENTS
26 June, 2009
p.giriraj:
dear sir, its a very good study,i would like to learn more from this study.i also like to do my project work in the same study. so please send me contact information to the crosponding author and more deatials regarding this thank you p.giriraj
07 July, 2009
Susanta Kumar ondal:
Dear Giriraj, Thanks for your comments. I am glad that the paper is useful to you. you may contact me for futher information. susanta_asw@yahoo.co.in Best wishes, Susanta
07 July, 2009
Susanta Kumar Mondal:
Thanks for your comments You may contact me at: susanta_asw@yahoo.co.in Best regards Susanta
 
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