In vitro Plant Regeneration Through Anther Culture of Five Rice Varieties

Research Article

In vitro Plant Regeneration Through Anther Culture of Five Rice Varieties

M. Asaduzzaman, M.A. Bari, M.H. Rahman, N. Khatun, M.A. Islam and M. Rahman

Anthers of five rice varieties viz. BR-5, BR-31, BR-34, BR-37 and BR-38 were cultured for callus induction and plant regeneration. Anthers were cultured on N6, Z2 and R2 media containing the same hormonal combination 2.5mg/l NAA, 0.5mg/l Kn and 0.5mg/l 2.4-D and incubated at 25 ± 1oC in dark for callus induction. All the varieties in Z2 medium, two varieties in N6 medium and only one variety in R medium produced callus. Out of all responding varieties BR-38 produced highest percentage of callus. Calli induced in different induction media were transferred to MS medium supplemented with 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l NAA and 1.0 mg/l Kn + 1.0 mg/l NAA and incubated at 25 ± 1^oC in light for plantlet regeneration. Green plantlets appeared within 15-30 days of culture and highest number of regenerated green (33.32%) and albino (11.27%) plantlets were produced in BR-37.

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M. Asaduzzaman, M.A. Bari, M.H. Rahman, N. Khatun, M.A. Islam and M. Rahman, 2003. In vitro Plant Regeneration Through Anther Culture of Five Rice Varieties . Journal of Biological Sciences, 3: 167-171.

DOI: 10.3923/jbs.2003.167.171

URL: https://scialert.net/abstract/?doi=jbs.2003.167.171

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Authors

M. Asaduzzaman

M.A. Bari

M.H. Rahman

N. Khatun

M.A. Islam

M. Rahman

Keywords

Research Article

In vitro Plant Regeneration Through Anther Culture of Five Rice Varieties

How to cite this article

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