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Journal of Biological Sciences
  Year: 2002 | Volume: 2 | Issue: 7 | Page No.: 438-440
DOI: 10.3923/jbs.2002.438.440
In vitro Shoot Development from Juvenile Cuttings of Field-Grown Olive (Olea europaea L.) cv. Leccino
Muhammad Ramzan Khan , Hamid Rashid and Azra Quraishi

Abstract:
Effects of different growth regulators on uni-nodal explants of field-grown olive (Olea europaea L.) cv. Leccino were investigated. Single-node cuttings were obtained from 8th bud pair. Disinfection protocols were standardized by using 0.1% mercuric chloride for five min followed by 50% Clorox for 15 min. sterilized cuttings were tested on MS medium supplemented with various plant growth regulators viz. gibberellic acid kinetin benzylaminopurine, and isopentenyladenine. These growth regulators were used singly in different combinations ranging from 1.0 to 8.0 μM l -1 In vitro. GA3 @ 4 μM l -1 was found to be more effective in enhancing shoot elongation. A higher concentration of kinetin, BAP and 2iP induced more callusing and hyperhydrated microshoots. Inclusion of polyvinylpyrrolidone @ 0.2 g/l in media was found to be successful to eliminate phenolic exudates.
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How to cite this article:

Muhammad Ramzan Khan , Hamid Rashid and Azra Quraishi , 2002. In vitro Shoot Development from Juvenile Cuttings of Field-Grown Olive (Olea europaea L.) cv. Leccino. Journal of Biological Sciences, 2: 438-440.

DOI: 10.3923/jbs.2002.438.440

URL: https://scialert.net/abstract/?doi=jbs.2002.438.440

 
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