Background and Objective: Avian Infectious Anemia is widely distributed around the world causing problems for both breeders and broilers. The transmission of this virus can be vertical or horizontal and is characterized by a clinical and subclinical presentation respectively. In order to prevent avian infectious anemia, specific titers are given to the breeder and then passed on to the chick. The purpose of this study was to examine the effectiveness of double vaccination in achieving higher and more uniform titers as well as decreasing the number of negative birds. Materials and Methods: The present study compared a traditional single-vaccine versus two other vaccination protocols using two doses in different combinations in three different flocks of Cobb×Cobb breeders. In this research serological evaluation was performed using the Idexx Avian Infectious Anemia Ab Test Laboratory kit. The breeder and the chick at birth was evaluated at different stages. Then they were compared utilizing various statistical methods according to the nature of the data. Results: Significant difference was found in the breeders at 20 weeks in favor of the protocols that used two doses and at 24 weeks in favor of the protocols that used one dose, however no difference was found in one-day-old chick. Conclusion: An additional vaccination against chicken anemia virus does not appear to be beneficial.
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