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International Journal of Pharmacology
  Year: 2017 | Volume: 13 | Issue: 6 | Page No.: 583-592
DOI: 10.3923/ijp.2017.583.592
 
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Effect of Danggui Buxue Tang on Erythropoiesis in Acute Chemotherapy Injured Mice
Junhua He, Zhaozhi Zeng, Jun Chen, Jinquan Wang, Qizhu Chen and Huaben Bo

Abstract:
Background and Objective: Hypocythemia is frequently developed in hematological and cancer patients. However its current therapies may induce the transmission of both viral and bacterial infection and lead to autoimmune pure red cell aplasia. The Chinese herbs exert their pharmacological effects through a multi-component and multi-target way coupled with its minimal side-effects. Danggui Buxue Tang (DBT) has been widely used for menopausal women as dietary supplements in China. This study aimed to evaluate the quality of Danggui Buxue Tang on the whole and the pharmacological effect from different levels. Materials and Methods: An HPLC method was established to develop chromatographic fingerprints of Danggui Buxue Tang from different areas. The fingerprints were analyzed with similarity evaluation system for chromatographic fingerprint of TCM. Comparison of peripheral blood count in different groups using HS-18 type fully automated hematology analyzer. Semi-solid medium was used to detect the number of erythroid progenitor cells. Erythroid cells numbers were detected by flow cytometry. The one-way analysis of variance (ANOVA) was used to determine statistical significance. Results: The experimental results showed that the fingerprints of the 6 samples of Danggui Buxue Tang had high similarity. So the mutual mode fingerprint for Danggui Buxue Tang was established and used for Danggui Buxue Tang quality control. However, only 17 common peaks in HPLC chromatogram were observed in 6 samples and the relative peak areas of common peaks were obviously different in different samples. Pharmacodynamic experiment showed that Danggui Buxue Tang could increase the number of peripheral reticulocyte, but had no effect on the number of peripheral red blood cells. Danggui Buxue Tang could increase the number of burst-forming unit-erythroid, colony forming unit-erythroid and erythroid cells in bone marrow. Conclusion: The fingerprint technique can be used for the quality evaluation and control of DBT. Danggui Buxue Tang can promote erythropoiesis in acute chemotherapy injured mice.
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How to cite this article:

Junhua He, Zhaozhi Zeng, Jun Chen, Jinquan Wang, Qizhu Chen and Huaben Bo, 2017. Effect of Danggui Buxue Tang on Erythropoiesis in Acute Chemotherapy Injured Mice. International Journal of Pharmacology, 13: 583-592.

DOI: 10.3923/ijp.2017.583.592

URL: https://scialert.net/abstract/?doi=ijp.2017.583.592

 
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