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International Journal of Pharmacology
  Year: 2010 | Volume: 6 | Issue: 3 | Page No.: 290-295
DOI: 10.3923/ijp.2010.290.295
Down-Regulation of Tyrosinase, TRP-2 and MITF Expressions by Neolitsea aciculata Extract in Murine B16 F10 Melanoma
S.S. Kim, W.J. Yoon, C.G. Hyun and N.H. Lee

Abstract:
Melanogenesis is a well known physiological response of human skin and is mainly caused by ultraviolet irradiation and some genetic factors. In our efforts to find new skin lightening agents, Neolitsea aciculata butanol fraction (NAB) was investigated for its ability to inhibit melanogenesis. In this study, NAB’s effect on inhibition of mushroom tyrosinase was assessed, which indicated its good anti-tyrosinase activity (IC50 211.4 Fg mL-1). Furthermore, NAB was shown to down-regulate melanin content on a dose dependent pattern. To clarify the target of its action in melanogenesis, Western blotting for tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2 and microphthalmia-associated transcription factor (MITF) was performed. As a result, NAB inhibited tyrosinase, TRP-2 and MITF expressions in a dose-dependent manner. However, it did not inhibit TRP-1 expression. In order to evaluate its potential in cosmetic applications, NAB’s cell toxicity was also determined by MTT assays using human dermal fibroblast cells. The NAB exhibited low cytotoxicity at 100 μg mL-1 in human cell line. Therefore, this study suggests that NAB is a potential anti-melanogenic agent and might be effective in treatment of hyperpigmentation disorders.
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How to cite this article:

S.S. Kim, W.J. Yoon, C.G. Hyun and N.H. Lee, 2010. Down-Regulation of Tyrosinase, TRP-2 and MITF Expressions by Neolitsea aciculata Extract in Murine B16 F10 Melanoma. International Journal of Pharmacology, 6: 290-295.

DOI: 10.3923/ijp.2010.290.295

URL: https://scialert.net/abstract/?doi=ijp.2010.290.295

 
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