
	Biotechnology
	Year: 2012 \| Volume: 11 \| Issue: 3 \| Page No.: 144-153 DOI: 10.3923/biotech.2012.144.153

Growth Parameters of Agaricus brasiliensis Mycelium on Wheat Grains in Solid-state Fermentation

H.S. Dalla-Santa, R. Rubel, F.M.D. Vitola, J.A. Rodriguez-Leon, O.R. Dalla-Santa, D. Brand, D.C. Alvarez, R.E.F. Macedo, J.C. Carvalho and C.R. Soccol

Abstract: Agaricus brasiliensis mycelium is rich in antitumoral molecules such proteoglycans and ergosterol and this is the first study to report production of mycelium and ergosterol by A. brasiliensis in solid-state fermentation. The aim of this research was to study the biomass content and several growth parameters of A. brasiliensis mycelium on grounded wheat grain as the substrate in solid-state fermentation with ergosterol as the main growth indicator. The growth parameters were determined by the respirometric activity in the columns of a bioreactor with forced aeration and the outlet air composition was determined by gas chromatography. Ergosterol was extracted, measured by liquid chromatography and used for calculating biomass production. Then, the respirometric activity and ergosterol production data were used to estimate biomass production with the program Fersol (_F). The growth parameters resulted in an achieved specific growth velocity of 0.016 h^-1 at 18 h and biomass yield (Y_X/O) = 0.160 g of biomass g^-1 of consumed O₂ at 302 h of fermentation. The analysis resulted in ergosterol values of 6.71 mg g^-1 of fungi biomass (Dry Weight: DW) from submerged fermentation and 1.95 mg g^-1 of solid-state-fermented wheat grains. The correlation with biomass production allowed us to estimate a value of 0.29 g g^-1 of fungi biomass per gram of the solid-state-fermented wheat grains. The importance of this study is allow calculation of the fungi biomass percentage in solid-state-fermented wheat grains and determination of the growth parameters of the main mycelium A. brasiliensis in this substrate, which can be milled to obtain a bio-flour to produce nutraceutical foods with beneficial effects due the presence of ergosterol which has recognized antitumor activity.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Relationship between Fungal Growth Morphologies and Ability to Secrete Lipase in Solid State Fermentation

•	Development of Bioprocess Engineering in China

•	Optimization of the Medium Composition for Production of Mycelial Biomass and Exo-Polysaccharide by Agaricus blazei Murill DPPh 131 Using Response-Surface Methodology

•	Lignocellulolytic Materials-as a Raw Material for the Production of Fermentable Sugars via Solid State Fermentation

How to cite this article:

H.S. Dalla-Santa, R. Rubel, F.M.D. Vitola, J.A. Rodriguez-Leon, O.R. Dalla-Santa, D. Brand, D.C. Alvarez, R.E.F. Macedo, J.C. Carvalho and C.R. Soccol, 2012. Growth Parameters of Agaricus brasiliensis Mycelium on Wheat Grains in Solid-state Fermentation. Biotechnology, 11: 144-153.

DOI: 10.3923/biotech.2012.144.153

URL: https://scialert.net/abstract/?doi=biotech.2012.144.153

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRICOLA
		AGRIS
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract

Science Alert