
	Biotechnology
	Year: 2007 \| Volume: 6 \| Issue: 4 \| Page No.: 489-496 DOI: 10.3923/biotech.2007.489.496

Screening, Isolation and Characterization of a Novel Antimicrobial Producing Actinomycete, Strain RAF10

Forar Laidi Rabah, Ali Elshafei, Mahmoud Saker, Bengraa Cheikh and Hacene Hocine

Abstract: A novel actinomycete strain designated RAF10, producing antimicrobial substances was isolated from an Egyptian soil. Morphological and chemical studies indicated that strain RAF10 belonged to the genus Streptomyces. The comparison of its physiological characteristics with those of known species of Streptomyces showed some differences with the nearest species Streptomyces enissocaesilis. Analysis of the 16S rDNA sequence of strain RAF10 showed a similarity level ranging between 97.22 and 98.37% within Streptomyces species, with S. enissocaesilis the most closely related. However, the phylogenetic analysis indicated that strain RAF10 represent a distinct phyletic line suggesting a new genomic species. This novel strain was active against Gram-positive and Gram-negative Bacteria, yeasts and filamentous fungi. The highest antibiotic formation was obtained when using (ISP 4) broth medium with some modifications, for 120 h at 28°C in New Brunswick Scientific Shaker at 200 rpm.

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How to cite this article:

Forar Laidi Rabah, Ali Elshafei, Mahmoud Saker, Bengraa Cheikh and Hacene Hocine, 2007. Screening, Isolation and Characterization of a Novel Antimicrobial Producing Actinomycete, Strain RAF10. Biotechnology, 6: 489-496.

DOI: 10.3923/biotech.2007.489.496

URL: https://scialert.net/abstract/?doi=biotech.2007.489.496

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