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Asian Journal of Biotechnology

Year: 2011 | Volume: 3 | Issue: 3 | Page No.: 237-245
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Authors

R. Rajesh Kannan

S.G. Prakash Vincent

Keywords

Research Article

Molecular Characterization of Antagonistic Streptomyces Isolated from a Mangrove Swamp

R. Rajesh Kannan and S.G. Prakash Vincent
The main objective of the present study was to isolate and identify the secondary metabolite producing Actinomycetes and to analyze the phylogenetic relationship. The soil samples were collected from the rhizosphre soil of Manakkudy mangroves, West Coast of India and screened for its antimicrobial potential. The novel bioactive compound producing strains were cloned in pGEM-T vector and sequenced. The phylogenetic relationship among the 20 Streptomyces sp. were studied. Phylogeny analysis showed that all the 20 isolates were having strong similarity (98%) with Streptomyces. Eight different species of Streptomyces such as Streptomyces olivoverticillatus, Streptomyces caelestis, Streptomyces roseoviridis, Streptomyces venezuelae, Streptomyces showdoensis, Streptomyces bikiniensis, Streptomyces griseoruber, Streptomyces roseus and 8 new strains (Streptomyces JS-1, Streptomyces JS-8, Streptomyces JS-10, Streptomyces JS-12, Streptomyces JS-14, Streptomyces JS-17, Streptomyces JS-19 and Streptomyces JS-20) were identified among the 20 isolates. The Streptomyces isolates JS-1, JS-8, JS-10, JS-12, JS-14, JS-17, JS-19 and JS-20 were considered as the new lineages of Streptomyces. Biochemical characterization was carried out and showed highly active by having different extracellular enzyme production to metabolize the nutrients in the environment. The GC contents were calculated for all the 20 Streptomyces isolates and it ranges between 58 and 59.6% in all the 20 sequences. All the isolates (JS-1 to JS-20) were having strong antagonistic activity against various bacterial and fungal pathogens, but the activity was differing in all the 20 Streptomyces. Phylogenetic analysis revealed that the isolates are the divergent of Streptomyces which are associated in the rhizosphere soil of Mangrove. A study of this kind will provide more details about the bioactive potential of Streptomyces from the estuarine ecosystem.
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Comments

baskaran Reply

i really admired about this paper and also it helps me to do further research

Rajesh Kannan Rajaretinam

Thanks for yor comment. All the best for your research career

Regards
Rajesh

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