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Biotechnology

Year: 2007 | Volume: 6 | Issue: 2 | Page No.: 184-192
Research Article

Cloning and Sequencing of Sucrose Synthase cDNA from Vegetable Soybean and its Expression During Storage at 20°C

Kassinee Sitthiwong
Faculty Of Agriculture, Kagawa University, Japan
Toshiyuki Matsui
Faculty Of Agriculture, Kagawa University, Japan
Nobuyuki Okuda
Faculty Of Agriculture, Kagawa University, Japan
Yusuke Kosugi
Faculty Of Agriculture, Kagawa University, Japan
Changes in carbohydrate content and the activity and gene expression of sucrose synthase (SS; EC 2.4.1.13) were studied in vegetable soybean (Glycine max (L.) Merr. cvs. ‘Ajigen’ and ‘Fuuki’) stored at 20°C for 10 days. After vegetable soybean is harvested, there was a progressive deterioration as shown in the reduction of sugars in both cultivars throughout the experimental period. In addition, SS activity decreased after 2 days of storage and increased with some fluctuations thereafter in both cultivars throughout the storage period. SS activity was higher in ‘Fuuki’ than ‘Ajigen’. To understand the molecular basis of induction of SS during posharvest senescence, the partial clone cDNA, GmSS (Glycine max sucrose synthase; AB213027) consisted of 607 nucleotides was isolated from vegetable soybean seeds. The GmSS gene showed highest identity at nucleotide and amino acid levels with mung bean at 94 and 98%, respectively. A radial phylogenetic tree of the amino acid sequence of GmSS (AB213027) and SS from mung bean (D10266) are strongly clustered in a subgroup, belonging to Fabaceae family. Although the level of transcripts was not consistent with enzyme activity, the expression was found in both cultivars throughout the experimental period. The inconsistency between the SS activity and gene expression may be due to post-transcriptional regulatory and/or encoded with another distinct isoforms of SS genes. Another result for this inconsistency between SS activity and gene expression could be due to the regulation of sugars by other harvest related genes.
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