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Asian Journal of Plant Pathology
  Year: 2011 | Volume: 5 | Issue: 3 | Page No.: 115-125
DOI: 10.3923/ajppaj.2011.115.125
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Diagnosis and Molecular Variability of Watermelon mosaic virus Isolates from North, East, North-east and North-west Regions of Iran
Zohreh Moradi

Watermelon Mosaic Virus (WMV) is a Potyvirus with a worldwide distribution. This virus causes serious economic losses in many cucurbits in Iran. To study relative incidence and molecular variability of WMV, 620 samples of cucurbit plants were collected from the different fields and cucumber greenhouses in some regions of Iran. Three hundred and three samples were infected by WMV in DAS-ELISA using specific polyclonal antibody (The rate of infection was 48.8%). In RT-PCR assay with specific primers of WMV, a single band of about 825 bp in length was produced from the samples. The Coat Protein (CP) region of the genome from 15 representative isolates were sequenced and compared with the sequences available in GeneBank. The identity of WMV CP nucleotide sequences of the 15 Iranian isolates ranged from 95.8 to 99.2%. According to this research Iranian isolates have high variation in CP gene. In most cases, geographical isolation is consistent with the phylogenetic grouping. Molecular weights of coat protein using SDS-PAGE were estimated at 34100 Da. Data shown in this study represent the occurrence of WMV based on ELIZA, RT-PCR and CP gene analyses in some region of Iran. These results make it possible to have a better understanding of the development of WMV disease in Iran.
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How to cite this article:

Zohreh Moradi , 2011. Diagnosis and Molecular Variability of Watermelon mosaic virus Isolates from North, East, North-east and North-west Regions of Iran. Asian Journal of Plant Pathology, 5: 115-125.

DOI: 10.3923/ajppaj.2011.115.125








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