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American Journal of Plant Physiology
  Year: 2018 | Volume: 13 | Issue: 2 | Page No.: 53-57
DOI: 10.3923/ajpp.2018.53.57
Adventitious and Denovo Somatic Embryogenesis from Nodal and Leaf Explants of Clerodendrum inerme
Sardar A. Farooq , Talat T. Farook and Salim H. Al Rawahy

Abstract:
Background and Objective: Clerodendrum inerme (L.) Gaertn. is commonly grown as a hedge plant in tropical countries, belongs to dicotyledon family Verbenaceae. It is used in the herbal medicine and has several useful phytochemicals, which can be exploited commercially without destruction of natural populations. To achieve large-scale micropropagation of plants, a rapid reproducible protocol is reported. Materials and Methods: Nodal and leaf explants were surface sterilized with 0.1% HgCl2 and plated on woody plant medium with three different concentrations of each auxin (2, 4-D and IBA); cytokinins (BAP and 2-ip) and a combination of these two. One way ANOVA is used and means are compared by Duncan’s multiple range test (p<0.01). Results: Adventitious (direct) organogenesis produced multiple shoots from nodal explants in media containing BAP while leaf explants yielded callus in IBA and 2, 4-D media. Denovo (indirect) differentiation of the callus produced somatic embryoids when transferred to cytokinin (BAP) media. Shoots were successfully rooted on PGR free media with reduced sucrose. About 25% mortality occurred in regenerated plants during acclimatization process. Conclusion: An efficient, rapid and reproducible plantlet regeneration protocol using nodal and leaf explants is reported for Clerodendrum inerme. Adventitious (direct) organogenesis from nodal explants produced multiple shoot buds within 4 weeks while denovo (indirect) somatic embryogenesis through callus is prevalent in leaf explants.
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How to cite this article:

Sardar A. Farooq, Talat T. Farook and Salim H. Al Rawahy, 2018. Adventitious and Denovo Somatic Embryogenesis from Nodal and Leaf Explants of Clerodendrum inerme. American Journal of Plant Physiology, 13: 53-57.

DOI: 10.3923/ajpp.2018.53.57

URL: https://scialert.net/abstract/?doi=ajpp.2018.53.57

 
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