American Journal of Plant Physiology1557-45391557-4547Academic Journals Inc.10.3923/ajpp.2018.53.57Clerodendrum inerme]]>A. FarooqSardar T. FarookTalat H. Al RawahySalim 22018132Background and Objective:Clerodendrum inerme (L.) Gaertn. is commonly grown as a hedge plant in tropical countries, belongs to dicotyledon family Verbenaceae. It is used in the herbal medicine and has several useful phytochemicals, which can be exploited commercially without destruction of natural populations. To achieve large-scale micropropagation of plants, a rapid reproducible protocol is reported. Materials and Methods: Nodal and leaf explants were surface sterilized with 0.1% HgCl2 and plated on woody plant medium with three different concentrations of each auxin (2, 4-D and IBA); cytokinins (BAP and 2-ip) and a combination of these two. One way ANOVA is used and means are compared by Duncans multiple range test (p<0.01). Results: Adventitious (direct) organogenesis produced multiple shoots from nodal explants in media containing BAP while leaf explants yielded callus in IBA and 2, 4-D media. Denovo (indirect) differentiation of the callus produced somatic embryoids when transferred to cytokinin (BAP) media. Shoots were successfully rooted on PGR free media with reduced sucrose. About 25% mortality occurred in regenerated plants during acclimatization process. Conclusion: An efficient, rapid and reproducible plantlet regeneration protocol using nodal and leaf explants is reported for Clerodendrum inerme. Adventitious (direct) organogenesis from nodal explants produced multiple shoot buds within 4 weeks while denovo (indirect) somatic embryogenesis through callus is prevalent in leaf explants.]]>Sudhersan, C., M. Abo El-Nil and J. Hussain,200354133147Pudutha, A., K. Venkatesh, P. Chakrapani, B.C.S. Singh, P. Kumar and A.R. Rani,2014Decalepis hamiltonii. Wight and Arn.]]>24268278Mounce, R., P. Smith and S. Brockington,2017Ex situ conservation of plant diversity in the world’s botanic gardens.]]>3795802Raven, P. and K. Havens,2014Ex situ plant conservation and cryopreservation: Breakthroughs in tropical plant conservation.]]>17512Canter, P.H., H. Thomas and E. Ernst,200523180185Chethana, G.S., K.R.H. Venkatesh and S.M. Gopinath,2013Clerodendrum inerme.]]>23840Gupta, S.P., S. Somkuwar and G. Garg,20108203212Baburaj, S., P. Ravichandran and M. Selvapandian,2000In vitro adventitious shoot formation from leaf cultures of Clerodendrum inerme (L.) Gaertn.]]>3812741276Farooq, S., T. Farook and S. Al-Rawahy,2010Clerodendrum inerme.]]>46S113S114Avani, K., P. Harish and S. Neeta,2006Ex situ conservation method for Clerodendrum inerme: A medicinal plant of India.]]>5415418Srinath, G., V. Sridhar and R. Renuka,2009In vitro propagaon of the medicinal plant-Clerodendrum inerme.]]>92123Nataraj, M., M.M. Kher and J.A.T. da Silva,2016Clerodendrum L. species: A review.]]>27169179Lloyd, G. and B. McCown,1980Kalmia latifolia, by use of shoot-tip culture.]]>30421426Bhatia, S., K. Sharma, R. Dahiya and T. Bera,2015Pages: 439Pages: 439Sharp, W.R., M.R. Sondahl, L.S. Caldas and S.B. Maraffa,1980in vitro asexual embryogenesis.]]>2268310Rengel, Z.,1987463343Abdul Mujib,2016Pages: 267Pages: 267Lu, D., W. Wei, W. Zhou, L.D. McGuigan and F.Y. Ji et al.,2017Castanea mollissima Blume).]]>130601616Zur, I., E. Dubas, M. Krzewska, P. Waligorski, M. Dziurka and F. Janowiak,2015Triticosecale Wittm.) anther cultures.]]>344762Pasternak, T., P. Miskolczi, F. Ayaydin, T. Meszaros, D. Dudits and A. Feher,200032129141Venkatachalam, P. and N. Jayabalan,1997Arachis hypogaea L.) by in vitro culture technology.]]>67237247Wahyuni, D.K., A.N.M. Ansori and F. Vidiyant,2014Justicia gendarussa Burm.f.]]>14668677D'Souza, M.C. and M. Sharon,2001In vitro clonal propagation of annatto (Bixa oreliana L.).]]>37168172Jana, S., I. Sivanesan and B.R. Jeong,2013in vitro multiplication of Sophora tonkinensis.]]>3549553Chen, R., M. Zhang, J. Lu, X. Zhang, J.A.T. da Silva and G. Ma,2014Valeriana jatamansi Jones.]]>165392397Muller, B. and J. Sheen,200845310941097Sane, D., A. Borgel, J.L. Verdeil and Y.K. Gassama,2000Acacia tortilis subsp. raddiana (Savi.) Brenan.]]>147257266