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Asian Journal of Crop Science
  Year: 2014 | Volume: 6 | Issue: 4 | Page No.: 305-319
DOI: 10.3923/ajcs.2014.305.319
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Development of a Plant Regeneration System from Seed-derived Shoot Segments of Rice (Oryza sativa L.)

W. Pilahome, S. Bunnag and A. Suwanagul

This study was carried out to establish a simple, efficient and reproducible protocol for plant regeneration in four rice genotypes which are Pokkali, RD6, Sakon Nakhon and Niaw Ubon 2. Two protocols were proposed and compared in this study, including (1) Multiple shoots induction and rooting and (2) Callus induction and regeneration. Single seeds of each cultivar were sown and germinated seed and shoot segments were used as explant source for the experiments. The results showed that a great number of multiple shoots induced from germinated seed could be achieved on MS solid medium supplemented with 50 μM 6-BAP alone, whereas rooting of the obtained shoots was easily induced on hormone-free MS solid medium. Compact callus formation could be induced from shoot segments on MS solid medium amended with 2 mg L-1 2,4-D alone, whilst a combination of 0.5 mg L-1 NAA and 2.0 mg L-1 6-BAP was most effective in promoting shoot regeneration from compact calli. Our study suggests that the regeneration frequency obtained via callus induction was inferior to that yielded through multiple shoots induction. The procedure reported here is expected to be useful in micropropagation and in vitro breeding and selection for drought and/or salt tolerance in rice for increasing the yield potential of this vital crop.
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How to cite this article:

W. Pilahome, S. Bunnag and A. Suwanagul, 2014. Development of a Plant Regeneration System from Seed-derived Shoot Segments of Rice (Oryza sativa L.). Asian Journal of Crop Science, 6: 305-319.

DOI: 10.3923/ajcs.2014.305.319






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