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American Journal of Biochemistry and Molecular Biology
  Year: 2020 | Volume: 10 | Issue: 2 | Page No.: 35-44
DOI: 10.3923/ajbmb.2020.35.44
 
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Regulation of Connective Components in Indomethacin-induced Gastric Ulcer Healing in Wistar Rats
S. Tijani Abiola , Fasawe M. Oluwaseun, Mrakpor O. Ono-jefe-eroro and Ebenezer O. Farombi

Abstract:
Background and Objective: There are growing evidences that non-steroidal anti-inflammatory drugs (NSAIDs)-induced depression of mitochondrial functions and activation of collagenase by lesions in the stomach. Therefore, this study sought to investigate possible mechanism of action of oleic acid rich fraction of n-hexane extract of Chasmanthera dependens (OHECD) in indomethacin-induced gastric ulcer. Materials and Methods: Gastric ulcer was induced using indomethacin orally. Gastric ulceration was assessed and gastric ulcer healing were analyzed by assessing the ulcer score, ulcer index, percentage healing, mucus content and levels of proline, hydroxyproline, nitric oxide and activities of pepsin and citrate synthase in the serum and gastric tissues. Results: Indomethacin (IND) caused severe gastric mucosa damage with ulcer score and ulcer index of 3.75±0.20 and 2.62±0.37 relative to control group at p<0.05, respectively. However, treatments with OHECD and standard drug cimetidine (CIM) significantly heal the ulcer with percentage ulcer healing of 61.60±0.42, 74.36±0.86 for different doses of OHECD and CIM, 68.15±0.20 respectively. Similarly, indomethacin administration decreased mucus content, proline, hydroxyproline, nitric oxide and citrate synthase activity with concomitant increase in pepsin activity, interestingly, OHECD or CIM significantly increased mucus content, proline, hydroxyproline, nitric oxide and citrate synthase activity with concomitant attenuation in pepsin activity. Conclusion: Overall, administration of OHECD stem promotes gastric ulcer healing by up regulating connective tissue components and recovery of mitochondrial function by increasing the mucus content, proline, hydroxyproline, nitric oxide and citrate synthase activity and decreased the peptic activity from drug-induced assault.
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How to cite this article:

S. Tijani Abiola, Fasawe M. Oluwaseun, Mrakpor O. Ono-jefe-eroro and Ebenezer O. Farombi, 2020. Regulation of Connective Components in Indomethacin-induced Gastric Ulcer Healing in Wistar Rats. American Journal of Biochemistry and Molecular Biology, 10: 35-44.

DOI: 10.3923/ajbmb.2020.35.44

URL: https://scialert.net/abstract/?doi=ajbmb.2020.35.44

 
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