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Asian Journal of Biochemistry

Year: 2011 | Volume: 6 | Issue: 3 | Page No.: 291-299
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Authors

H.A. Bankole

O.A. Magbagbeola

O.B. Adu

A.A. Fatai

B.A. James

Keywords

Research Article

Biochemical Effect of Ethanolic Extract of Phyllanthus amarus (Euphorbiaceae) on Plasma Nitric Oxide and Penile Cyclic Guanosine Monophosphate (cGMP) in Mature Male Guinea Pigs

H.A. Bankole, O.A. Magbagbeola, O.B. Adu, A.A. Fatai and B.A. James
The aim of this study was to investigate the probable mode of action of ethanolic extract of Phyllanthus amarus traditionally used to correct erectile dysfunction by monitoring the effect on the concentration of penile cyclic guanosine mono-phosphate (cGMP) and the concentration of plasma Nitric Oxide (NO) in male guinea pigs that were administered different doses (100, 200 and 400 mg kg-1 body weight) of the plant extract using direct immunoassay kits. Physiological saline and sildenafil were given to animals that served as the controls. Phytochemical screening of the plant determined included alkaloids, flavonoids, terpenoids, saponins, tannins, steroids and cardiac glycosides. There was no statistical significant difference in the weight of the animals before and after the administration of the plant extract. The penile weight per body weight ratio showed a significant difference between groups (p<0.05). There was a dose dependent increase in the level of cGMP in the different groups administered different doses of the extract, with the 400 mg kg-1 group showing significant difference compared to lower-dose and saline groups (p<0.05). Plasma nitric oxide concentration was not significantly different among the control and experimental groups. Conclusively, ethanol extract of the plant had a dose dependent increase on the level of cGMP which might account for the claim of the plant been use as a sexual stimulant and the increase level of the cGMP probably had a feedback effect on nitric oxide synthase which resulted to reduced concentration of NO in the plasma.
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How to cite this article

H.A. Bankole, O.A. Magbagbeola, O.B. Adu, A.A. Fatai and B.A. James, 2011. Biochemical Effect of Ethanolic Extract of Phyllanthus amarus (Euphorbiaceae) on Plasma Nitric Oxide and Penile Cyclic Guanosine Monophosphate (cGMP) in Mature Male Guinea Pigs. Asian Journal of Biochemistry, 6: 291-299.

DOI: 10.3923/ajb.2011.291.299

URL: https://scialert.net/abstract/?doi=ajb.2011.291.299

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