
	Asian Journal of Applied Sciences
	Year: 2014 \| Volume: 7 \| Issue: 7 \| Page No.: 621-634 DOI: 10.3923/ajaps.2014.621.634

Antimicrobial activity of Enterococcus faecium NM₂ Isolated from Urine: Purification, Characterization and Bactericidal Action of Enterocin NM₂

Gamal Enan, Abdul-Raouf Al- Mohammadi, Gamal El- Didamony, Mahmoud E.F. Abdel- Haliem and Azza Zakaria

Abstract: Cell Free Supernatants (CFS) containing bacteriocin of Enterococcus faecium NM₂ (E. faecium NM₂) isolated from urine inhibited many gram-positive and gram-negative pathogenic bacteria. It also inhibited the Candida albicans M₂ fungus. The antibiotic sensitivity test of the indicator bacteria showed that these strains were resistant to 60-75% of the antibiotics used. The E. faecium NM₂ bacteriocin was purified by ammonium sulphate precipitation and gel filtration and a 3600 fold-increase in specific activity of bacteriocin was obtained. The purified bacteriocin showed an apparent molecular mass of Ca, 5 KDa. Amino acid analysis showed that the E. faecium NM₂ bacteriocin consists of 16 amino acids with high content of glycine, alanine, glutamic acid and asparagine. The E. faecium NM₂ bacteriocin was designated enterocin NM₂ and showed a bactericidal action on sensitive bacterial strains used. Enterocin NM₂ could be classified as a novel variant within class IIc bacteriocins.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Prevalence, Characterization and Inhibition by Probiotics of Multidrug Resistant Bacteria Isolated from Renal Failure Patients Undergoing Hemodialysis

•	Isolation and Molecular Identification of Food Grade Lactic Acid Bacteria and Their Antifungal Activity

•	Characterization and Identification of Multidrug Resistant Bacteria from Some Egyptian Patients

•	Behaviour of Listeria monocytogenes LMG 10470 in Poultry Meat and its Control by the Bacteriocin Plantaricin UG 1

•	Inhibition of Clostridium perfringens LMG 11264 in Meat Samples of Chicken Turkey and Beef by the Bacteriocin Plantaricin UG1

•	Novel Antibacterial Activity of Enterococcus faecium NM₂ Isolated from Urine of Healthy People

How to cite this article:

Gamal Enan, Abdul-Raouf Al- Mohammadi, Gamal El- Didamony, Mahmoud E.F. Abdel- Haliem and Azza Zakaria, 2014. Antimicrobial activity of Enterococcus faecium NM₂ Isolated from Urine: Purification, Characterization and Bactericidal Action of Enterocin NM_{2. Asian Journal of Applied Sciences, 7: 621-634.

DOI: 10.3923/ajaps.2014.621.634

URL: https://scialert.net/abstract/?doi=ajaps.2014.621.634}

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Google Scholar

Science Alert

Antimicrobial activity of Enterococcus faecium NM2 Isolated from Urine: Purification, Characterization and Bactericidal Action of Enterocin NM2

Antimicrobial activity of Enterococcus faecium NM₂ Isolated from Urine: Purification, Characterization and Bactericidal Action of Enterocin NM₂