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Biotechnology
  Year: 2013 | Volume: 12 | Issue: 2 | Page No.: 65-73
DOI: 10.3923/biotech.2013.65.73
 
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Characterization and Identification of Multidrug Resistant Bacteria from Some Egyptian Patients

Seham Abdel-Shafi, Sahar M. Ouda, Ibrahim Elbalat and Gamal Enan

Abstract:
The isolation of multidrug resistant bacteria from Egyptian patients showed a great interest to study such phenomenon. Hence, simple methods were followed herein to isolate and characterize the antibiotic resistant variants by the common phenotypic, morphological and biochemical characters. Out of 500 clinical bacterial cultures, 50 only were multidrug resistant bacteria with a value of drug resistance ability of about 10%. About 46% of multidrug resistant bacterial cultures tested were isolated from urine samples. The percentage values of both resistance and susceptibility of the 50 multidrug resistant bacterial isolates to 14 types of antibiotics were calculated. Based on their cultural, morphological and biochemical characteristics, the 50 multidrug resistant bacterial isolates were identified and categorized into eight groups. The identified bacterial species were arranged in a descending order according to their frequency percentage viz. Escherichia coli>Staphylococcus aureus> Pseudomonas aeruginosa> Klebsiella pneumoniae>Streptococcus pyogenes> Proteus vulgaris>Streptococcus pneumoniae> Staphylococcus saprophyticus. The relationship between pathogenic cases, symptoms and the identified multidrug bacterial pathogens was studied. A simple key was designed for easy differentiation and classification of the 50 multidrug resistant bacterial organisms. It was based on easily determinable characteristics which were used for rapid assignment of bacteria into genera and species.
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How to cite this article:

Seham Abdel-Shafi, Sahar M. Ouda, Ibrahim Elbalat and Gamal Enan, 2013. Characterization and Identification of Multidrug Resistant Bacteria from Some Egyptian Patients. Biotechnology, 12: 65-73.

DOI: 10.3923/biotech.2013.65.73

URL: https://scialert.net/abstract/?doi=biotech.2013.65.73

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