Wilson Abrao Saad
Basic Institute of Biosciences-UNITAU, Taubate, SF, Brazil
Ismael Francisco Motta Siqueira Guarda
Department of Anesthesiology, Clinic Hospital State of Sao Paulo Sao Paulo, Brazil
Luiz Antonio de Arruda Camargo
Department of Physiology and Pathology, School of Dentistry,
Paulista State University, UNESP Araraquara, SF Brazil
Talmir Augusto Faria Brizola dos Santos
Basic Institute of Biosciences-UNITAU, Taubate, SF, Brazil
Sylvio Simoes
Basic Institute of Biosciences-UNITAU, Taubate, SF, Brazil
William Abrao Saad
Department of Gastroenterology, Clinic Hospital State of Sao Paulo Sao Paulo, Brazil
ABSTRACT
The aim of this study was to examine the role of nifedipine and Nitric Oxide (NO) on salivary flow and compounds (salivary amylase, saliva total proteins, saliva calcium, sodium and potassium). Male Holtzman rats weighting 200-250 g were anesthetized with zoletil 50 mg kg-1 (tiletamine chloridrate 125.0 mg and zolazepan chloridrate 125.0 mg) into quadriceps muscle and stainless steel cannulas were implanted into their lateral ventricle of the brain (LV). Animals in divided group were injected with nifedipine (50 μg μL-1) alone and in combination with 7-nitroindazol (7-NIT) (40 μg μL-1), neuronal NO Sinthase Inhibitor (nNOSI) and Sodium Nitroprussate (SNP) (30 μg μL-1) NO donor agent. As a secretory stimuli, pilocarpine dissolved in isotonic was administered intraperitoneally (ip) at a dosage of 10 mg kg-1 body weight. Saliva was collected for 7 min with four cotton balls weighing approximately 20 mg each, two of which were placed on either side of the oral cavity, with the other two placed under the tongue. Nifedipine treatment induced a reduction in saliva secretion rate and concentration of amylase, total protein and calcium without changes in sodium and potassium concentration in comparison with controls. Co-treatment of animals with nifedipine and SNP retained flow rate and concentration of amylase, total protein and calcium in normal levels. Co-treatment of animals with nifedipine and 7-NIT potentiated the effect of nifedipine on the reduction of saliva secretion and concentrations of amylase, total protein and calcium. Nifedipine (dihydroperidine) calcium-channel blocker widely in use is associated with salivary dysfunction acting in the central nervous system structures. NO might be the mechanism for protective effect against the nifedipine-induce salivary dysfunction, acting in the CNS.
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How to cite this article
Wilson Abrao Saad, Ismael Francisco Motta Siqueira Guarda, Luiz Antonio de Arruda Camargo, Talmir Augusto Faria Brizola dos Santos, Sylvio Simoes and William Abrao Saad, 2006. Central Nifedipine-induced Alterations in Salivary Flow and Compounds: Role of Nitric Oxide. Journal of Biological Sciences, 6: 596-603.
DOI: 10.3923/jbs.2006.596.603
URL: https://scialert.net/abstract/?doi=jbs.2006.596.603
DOI: 10.3923/jbs.2006.596.603
URL: https://scialert.net/abstract/?doi=jbs.2006.596.603
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