Beng Erda
Department of Biology, Faculty of Science and Arts, Adnan Menderes University, 09010 Aydin, Turkey
Yelda Emek
Department of Biology, Faculty of Science and Arts, Adnan Menderes University, 09010 Aydin, Turkey
ABSTRACT
In vitro adventitious shoot regeneration of Liquidambar orientalis Miller (Anatolian sweet gum) endemic to Turkey was studied using leaf explants to provide an efficient alternative propagation method. Sterile leaf explants, harvested from shoot proliferating cultures, were placed on solidified Woody Plant Medium (WPM) supplemented with 0 or 0.54 μM -naphthaleneacetic acid (NAA) and various concentrations of 6-benzylaminopurine (BAP). Shoot organogenesis occurred directly, without a distinct callus stage and well-defined shoots were visible between 6th and 9th weeks. The highest shoot numbers were achieved on WPM supplemented with 0.54 μM NAA and 11.1 μM BAP (19.97 shoot/explant). Shoots were elongated on WPM with 3.3 μM BAP. Excised shoots (2-3 cm) were rooted on the WPM with addition of 9.8 μM indole-3-butyric acid (IBA) (83.3%). About 80% of the rooted shoots were established in pots after hardening.
PDF References Citation
How to cite this article
Beng Erda and Yelda Emek, 2005.
In vitro Adventitious Shoot Regeneration of Liquidambar orientalis Miller. Journal of Biological Sciences, 5: 805-808.
DOI: 10.3923/jbs.2005.805.808
URL: https://scialert.net/abstract/?doi=jbs.2005.805.808
REFERENCES
- Fay, M.F., 1992. Conservation of rare and endangered plants using in vitro methods. In Vitro Cell. Dev. Biol. Plant, 28: 1-4.
CrossRefDirect Link - Krogstrup, P., S. Baldursson and J.V. Norgaard, 1992. Ex situ genetic conservation by use of tissue culture. Operat. Bot., 113: 49-53.
Direct Link - Fay, M.F., 1994. In what situations is in vitro culture appropriate to plant conservation? Biodiversity Conserv., 3: 176-183.
CrossRefDirect Link - Cuenca, S., J.B.A. Marco and R. Parra, 1999. Micropropagation from inflorescence stems of the Spanish endemic plant Centaurea paui Loscos ex Willk. (Compositae). Plant Cell Rep., 18: 674-679.
CrossRefDirect Link - Lee, S.K. and A.N. Rao, 1986. In vitro regeneration of plantlets in Fagraea fragrans Roxb a tropical tree. Plant Cell Tissue Organ Culture, 7: 43-51.
CrossRef - Lloyd, G. and B. McCown, 1980. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Proc. Int. Plant Propagator's Soc., 30: 421-426.
Direct Link - Brand, M.H. and R.D. Lineberger, 1991. The effect of leaf source and developmental stage on shoot organogenic potential of sweet gum (Liquidambar styraciflua L.) leaf explants. Plant Cell Tissue Organ Culture, 24: 1-7.
CrossRef