Rahman Md. Mukhlesur
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
Yutaka Hirata
Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo 183-8509, Japan
Shah-E-Alam
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
ABSTRACT
Genetic relationship were evaluated among thirty two cultivars of Brassica rapa, collected from Bangladesh, Japan and China, by employing SDS-PAGE for seed protein and esterase, acid phosphate and peroxidase analysis. SDS-PAGE generated a total of 32 bands of which nearly 31% were polymorphic, followed by esterase of 9 bands of nearly 19% polymorphic and no identifyable polymorphic band was found from both acid phosphate and esterase analysis. However, these polymorphic markers clearly distinguished between yellow sarson and brown seeded cultivars. Cluster analysis using data generated by SDS-PAGE for seed protein, clearly separated the yellow sarson, self-compatible cultivars from the brown seeded, self-incompatible cultivars, as well as different brown seeded cultivars from Bangladesh, Japan and China origin.
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How to cite this article
Rahman Md. Mukhlesur, Yutaka Hirata and Shah-E-Alam, 2004. Genetic Variation Within Brassica rapa Cultivars Using SDS-PAGE for Seed Protein and Isozyme Analysis. Journal of Biological Sciences, 4: 239-242.
DOI: 10.3923/jbs.2004.239.242
URL: https://scialert.net/abstract/?doi=jbs.2004.239.242
DOI: 10.3923/jbs.2004.239.242
URL: https://scialert.net/abstract/?doi=jbs.2004.239.242
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