Khurshid Anwar
Department of Zoology, University of Azad Jammu and Kashmir, Muzaffarabad, Azad Kashmir, Pakistan
ABSTRACT
Toxicity of permethrin was investigated in the liver of newly hatched chicks developed from the eggs injected with a single sublethal doses (0.05 ml) of three different concentrations of permethrin insecticide (50, 100 and 200ppm) on day 0 of incubation. Study included the estimation of a few enzyme activities and some biochemical constituents of liver. Among enzymes, the activities of amylase, alkaline phosphatase, acid phosphatase, glutamate oxaloacetate transaminase were significantly decreased whereas the activity of lactate dehydrogenase was increased. However, the activity of glutamate pyruvate transaminase remained unchanged. From among biochemical components total protein, total lipids and cholesterol were significantly elevated whereas urea and DNA contents were significantly reduced. Glucose, glycogen, soluble protein, free amino acids (FAA), uric acid and RNA contents remained unaltered. Permethrin-induced common histopathological changes observed were disturbance in the hepatic architecture, increased sinusoidal spaces, cytoplasmic vacuolations, hepatocytic nuclear condensation and fragmentation, hydropic degeneration and necrosis and the presence of anucleated hepatocytes. A mild blood cell infiltration in the hepatic parenchyma was observed.
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Khurshid Anwar, 2003. Toxicological Effect of Single Treatment of Permethrin Injected into the Eggs on 0 Day of Incubation on the Liver of Newly Hatched Chick. Journal of Biological Sciences, 3: 660-673.
DOI: 10.3923/jbs.2003.660.673
URL: https://scialert.net/abstract/?doi=jbs.2003.660.673
DOI: 10.3923/jbs.2003.660.673
URL: https://scialert.net/abstract/?doi=jbs.2003.660.673
REFERENCES
- Abdel-Rehman, A., A.K. Shetty and M.B. Abou-Donia, 2001. Subchronic dermal application of N,N-diethyl-m-toluamide (DEET) and permethrin to adult rats, alone or in combination, causes diffuse neuronal cell death and cytoskeletal in the cerebral cortex and hippocampus and purkinje neuron loss in the cerebellum. Exp. Neurol., 172: 153-171.
PubMedDirect Link - Abuelgasim, A., R. Ringer and V. Sanger, 1981. Toxicosis of Mirex for chick embryos and chickens hatched from eggs. Avian. Dis., 26: 34-39.
PubMedDirect Link - Abu-Qare, A.W. and M.B. Abou-Donia, 2001. Simultaneous determination of chlorpyrifos, permethrin and their metabolites in rat plasma and urine by high-performance liquid chromatography. Anal. Toxicol., 25: 275-279.
Direct Link - Akhtar, M.H., C. Danis, H.L. Trenholm and K.E. Hartin, 1992. Deltamethrin residues in milk and tissues of lactating dairy cows. J. Environ. Sci. Health Part B: Pestic. Food Contam. Agric. Wastes, 27: 235-253.
CrossRefPubMedDirect Link - Cabaud, P.G. and F. Wroblewski, 1958. Colorimetric measurement of lactic dehydrogenase activity of body fluids. Am. J. Clin. Pathol., 30: 234-236.
PubMedDirect Link - Carafoli, E., 1987. Intracellular calcium homeostasis. Ann. Rev. Biochem., 56: 395-433.
PubMedDirect Link - Chouela, E., A. Abeldano, G. Pellerano and M.I. Hernandez, 2002. Diagnoses and treatment of scabies: A practical guide. Am. J. Clin. Dermatol., 3: 9-18.
PubMedDirect Link - Deichmann, W.B., I. Dressler, M. Keplinger and W.E. Macdonald, 1968. Retention of the dieldrin in blood, liver and fat of rats fed dieldrin for 6 month. Indian. Med. Surg., 37: 837-839.
PubMedDirect Link - Ishmael, J.M. and M.H. Lithfield, 1988. Chronic toxicity and carcinogenic evaluation of permethrin in rats and mice. Fundam. Applied Toxicol., 11: 308-322.
PubMed - Kapoor, R.K., S.S. Chauhan, N. Sing and U.K. Misra, 1988. Induction of hepatic mixed-function oxidases by permethrin and cypermethrin in chicks fed vitamin A-deficient diet. Pestic. Biochem. Physiol., 32: 205-211.
CrossRefDirect Link - Kind, P.R.N. and E.J. King, 1954. Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J. Clin. Pathol., 7: 322-326.
CrossRefPubMedDirect Link - Kostka, G., D. Palut and B. Wiadrowska, 1997. The effect of permethrin and DDT on the activity of cytochrome P-450 1A and 2B molecular forms in rat liver. Rocz Panstw Zakl Hig., 48: 229-237.
PubMedDirect Link - Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall, 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem., 193: 265-275.
CrossRefPubMedDirect Link - Majmunder, S., A.K. Chakraborty, T.K. Mandal, A. Bhattacharya and K. Basak, 1994. Subacute toxicity of fenvalerate in broiler chicks: Concentration, cytotoxicity and biochemical profiles. Indian J. Exp. Biol., 32: 752-756.
PubMedDirect Link - Mannervik, B. and U.H. Danielson, 1988. Glutathione transferases-structure and catalytic activity. Crit. Rev. Biochem., 23: 283-337.
PubMedDirect Link - Moore, S. and W.H. Stein, 1954. A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. J. Biol. Chem., 211: 907-913.
CrossRefDirect Link - Natelson, S., M.L. Scott and C. Beffa, 1951. A rapid method for the estimation of urea in biologic fluids. Am. J. Chem. Pathol., 21: 275-281.
PubMedDirect Link - Ray, S.D., V.R. Mumaw, R.R. Raje and M.W. Fariss, 1996. Protection of acetaminophen-induced hepatocellular apoptosis and necrosis by cholesteryl hemisuccinate pretreatment. J. Pharmacol. Exp. Ther., 279: 1470-1483.
PubMed - Reitman, S. and S. Frankel, 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol., 28: 56-63.
CrossRefPubMedDirect Link - Scheideler, S.E., 1993. Effects of various types of aluminosilicates and aflatoxin B1 on aflatoxin toxicity, chick performance and mineral status. Poult. Sci., 72: 282-288.
CrossRefPubMedDirect Link - Sheets, L.P., 2000. A consideration of age-dependent differences in susceptibility to organophosphorus and pyrethroid insecticides. Neurotoxicology, 21: 57-63.
PubMedDirect Link - Sibley, P.K. and N.K. Kaushik, 1991. Toxicity of microencapsulated permethrin to selected nontarget aquatic invertebrates. Arch. Environ. Contam. Toxicol., 20: 168-176.
CrossRefDirect Link - Spencer, F. and Z. Berhane, 1982. Uterine and fetal characteristics in rats following a post-implantational exposure to permethrin. Bull. Environ. Contam. Toxicol., 29: 84-88.
CrossRefDirect Link - Vontas, J.G., G.J. Small and J. Hemingway, 2001. Glutathione-s-transferases as antioxidant defence agents confer pyrethroid resistance in Nilaparvata lugens. Biochem. J., 357: 65-72.
PubMedDirect Link - Walker, N.E., 1971. The effect of malathion and malaoxon on esterases and gross development of the chick embryo. Toxicol. Applied Pharmacol., 19: 590-601.
CrossRefPubMedDirect Link - Schneider, W.C., 1957. Determination of Nucleic Acids in Tissues by Pentose Analysis. In: Methods in Enzymology, Colowick, S.P. and N.O. Kaplan (Eds.). Vol. 3, Academic Press, New York, USA., pp: 680-684.
Direct Link - Karen, D.J., W. Li, P.R. Harp, J.S. Gillette and J.R. Bloomquist, 2001. Striatal dopaminergic pathways as a target for the insecticide permethrin and chloropyrifos. Neurotoxicology, 22: 811-817.
CrossRef