Shah, H.
National Agricultural Research Center, lslamabad-45500, Pakistan
S. Khalid
National Agricultural Research Center, lslamabad-45500, Pakistan
I. Ahmad
National Agricultural Research Center, lslamabad-45500, Pakistan
ABSTRACT
To ascertain the prevalence and distribution of four major pepper viruses viz. chili veinal mottle potyvirus (CVMV), cucumber mosaic cucmovirus (CMV), tobacco mosaic tobamovirus (TMV) & potato virus Y (PVY). Surveys of chili crop in three major provinces of Pakistan were conducted during 1996-98. At each location 3-5 farmer`s fields were inspected and samples showing virus-like symptoms were collected. A total of 500 samples (200 Punjab, 200 Sindh and 100 NWFP) were collected and tested through direct double antibody sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA). CVMV & CMV appeared as the most prevalent viruses in almost all the surveyed areas. In Sindh, the incidence of CVMV, CMV, TMV, and PVY was 9, 16, 5 and 3.5%, respectively. In Punjab, CVMV appeared as the most important virus (63%) infecting pepper crop in all districts followed by CMV (20%). Co-infection of CVMV and CMV (10%) was recorded in Faisalabad, Multan and Darya Khan. In most CMV positive samples, TMV or CVMV or PVY were also detected (co-infection). In NWFP, CVMV incidence was 50% followed by CMV (17.8%). On the average, survey data showed that CVMV (40.6% incidence) is still the most prevalent virus infecting pepper followed by CMV (17.8%) in these three provinces. PVY was only detected in Sindh (3.5%) and NWFP (6%). TMV was not detected in any place of NWFP and Punjab, but detected in mixed infection in Sindh (5%). Thus there is 19.6% increase in CVMV incidence while frequency of other three viruses remained the same as compared to previous reports.
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How to cite this article
Shah, H., S. Khalid and I. Ahmad, 2001. Prevalence and Distribution of Four Pepper Viruses in Sindh, Punjab and
North West Frontier Province. Journal of Biological Sciences, 1: 214-217.
DOI: 10.3923/jbs.2001.214.217
URL: https://scialert.net/abstract/?doi=jbs.2001.214.217
DOI: 10.3923/jbs.2001.214.217
URL: https://scialert.net/abstract/?doi=jbs.2001.214.217
REFERENCES
- Clark, M.F. and A.N. Adams, 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol., 34: 475-483.
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