Jacob Coppedge
Department of Poultry Science, Texas A&M University, College Station, TX 77843-2472, USA
Joseph Klein
Department of Poultry Science, Texas A&M University, College Station, TX 77843-2472, USA
Blyn Brown
Department of Poultry Science, Texas A&M University, College Station, TX 77843-2472, USA
Brent Ratliff
Enzyvia LLC, Sheridan, IN 46069, USA
Frank Ruch
Enzyvia LLC, Sheridan, IN 46069, USA
Jason T. Lee
Enzyvia LLC, Sheridan, IN 46069, USA
ABSTRACT
An experiment was conducted to evaluate the effect of varying levels of phytase with and without NSPase inclusion on broiler performance when supplemented in corn/soy bean meal diets low in available phosphorus. The objective was to determine if NSPase inclusion enhances phytase activity in relation to growth parameters and bone ash in broilers reared in batteries through 14 days of age. Four diets with selected available phosphorus levels of 0.15, 0.20%, 0.25% and 0.30% were included in the experimental design to develop a dose response curve to calculate phosphorus release from experimental treatments. An addition six treatments were included that included three levels of phytase (150, 200 and 250 FTU/kg) with and without NSPase inclusion in a diet containing 0.15% available phosphorus. Evaluated parameters included body weight, feed conversion ratio and bone ash percentage. Body weight and bone ash percentage were positively influence with increases in available phosphorus levels. Phytase inclusion positively influenced growth performance and bone ash percentage. Broilers fed the 200 and 250 FTU/kg phytase inclusion levels outperformed the broilers fed the 150 FTU/kg inclusion level. Addition of NSPase with 150 FTU/kg phytase resulted in increased broiler body weight as compared to the 150 FTU/kg phytase diet alone. Using regression equations determined from dose response treatments for body weight, bone ash (mg) and bone ash percentage, NSPase inclusion increase phosphorus release at the 150 FTU/kg level from a 0.06% to 0.09%. These data indicate that NSPase inclusion may increase phytase effectiveness when co-administered during early stages of growth.
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How to cite this article
Jacob Coppedge, Joseph Klein, Blyn Brown, Brent Ratliff, Frank Ruch and Jason T. Lee, 2011. Effects of Co-administration of Phytase and NSPase on Broiler Performance and Bone Ash. International Journal of Poultry Science, 10: 933-939.
DOI: 10.3923/ijps.2011.933.939
URL: https://scialert.net/abstract/?doi=ijps.2011.933.939
DOI: 10.3923/ijps.2011.933.939
URL: https://scialert.net/abstract/?doi=ijps.2011.933.939
REFERENCES
- Bedford, M.R. and A.J. Morgan, 1996. The use of enzymes in poultry diets. World's Poult. Sci. J., 52: 61-68.
CrossRefDirect Link - Choct, M., R.J. Hughes and M.R. Bedford, 1999. Effects of a xylanase on individual bird variation, starch digestion throughout the intestine and ileal and caecal volatile fatty acid production in chickens fed wheat. Br. Poult. Sci., 40: 419-422.
CrossRefDirect Link - Cowieson, A.J. and O. Adeola, 2005. Carbohydrases, protease and phytase have an additive beneficial effect in nutritionally marginal diets for broiler chicks. Poult. Sci., 84: 1860-1867.
CrossRefPubMedDirect Link - Dilger, R.N., E.M. Onyango, J.S. Sands and O. Adeola, 2004. Evaluation of microbial phytase in broiler diets. Poult. Sci., 83: 962-970.
Direct Link - Francesch, M. and P.A. Geraert, 2009. Enzyme complex containing carbohydrases and phytase improves growth performance and bone mineralization of broilers fed reduced nutrient corn-soybean-based diets. Poult. Sci., 88: 1915-1924.
CrossRefPubMedDirect Link - Gao, F., Y. Jiang, G.H. Zhou and Z.K. Han, 2007. The effects of xylanase supplementation on growth, digestion, circulating hormone and metabolite levels, immunity and gut microflora in cockerels fed on wheat-based diets. Br. Poult. Sci., 48: 480-488.
CrossRefDirect Link - Ghorbani, M.R., J. Fayazi and M. Chaji, 2009. Effect of dietary phytase and NSP-degrading enzymes in diets containing rape seed meal on broiler performance and carcass characteristic. Res. Biol. Sci., 4: 258-264.
Direct Link - Gracia, M.I., M.J. Aranibar, R. Lazaro, P. Medel and G.G. Mateos, 2003. Alpha-amylase supplementation of broiler diets based on corn. Poult. Sci., 82: 436-442.
CrossRefPubMedDirect Link - Juanpere, J., A.M. Perez-Vendrell, E. Angulo and J. Brufau, 2005. Assessment of potential interactions between phytase and glycosidase enzyme supplementation on nutrient digestibility in broilers. Poult. Sci., 84: 571-580.
CrossRefPubMedDirect Link - Kocher, A., M. Choct, M.D. Porter and J. Broz, 2002. Effects of feed enzymes on nutritive value of soybean meal fed to broilers. Br. Poult. Sci., 43: 54-63.
CrossRefDirect Link - Kumar, V., A.K. Sinha, H.P.S. Makkar and K. Becker, 2010. Dietary roles of phytate and phytase in human nutrition: A review. Food Chem., 120: 945-959.
CrossRefDirect Link - Lazaro, R., M. Garcia, M.J. Aranibar and G.G. Mateos, 2003. Effect of enzyme addition to wheat-, barley- and rye-based diets on nutrient digestibility and performance of laying hens. Br. Poult. Sci., 44: 256-265.
CrossRefPubMedDirect Link - Lee, J.T., C.A. Bailey and A.L. Cartwright, 2003. Guar meal germ and hull fractions differently affect growth performance and intestinal viscosity of broiler chickens. Poult. Sci., 82: 1589-1595.
CrossRefDirect Link - Lee, J.T., C.A. Bailey and A.L. Cartwright, 2003. β-mannanase ameliorates viscosity-associated depression of growth in broiler chickens fed guar germ and hull fractions. Poult. Sci., 82: 1925-1931.
CrossRefDirect Link - Meng, X. and B.A. Slominski, 2005. Nutritive values of corn, soybean meal, canola meal and peas for broiler chickens as affected by a multicarbohydrase preparation of cell wall degrading enzymes. Poult. Sci., 84: 1242-1251.
CrossRefDirect Link - Nelson, T.S., T.R. Shieh, R.J. Wodzinski and J.H. Ware, 1971. Effect of supplemental phytase on the utilization of phytate phosphorus by chicks. J. Nutr., 101: 1289-1293.
CrossRefPubMedDirect Link - Powell, S., S. Johnston, L. Gaston and L.L. Southern, 2008. The effect of dietary phosphorus level and phytase supplementation on growth performance, bone-breaking strength and litter phosphorus concentration in broilers. Poult. Sci., 87: 949-957.
CrossRefDirect Link - Rao, D.E.C., K.V. Rao, T.P. Reddy and V.D. Reddy, 2009. Molecular characterization, physiochemical properties, known and potential applications of phytases: An overview. Crit. Rev. Biotechnol., 29: 182-198.
PubMed - Ravindran, V., W.L. Bryden and E.T. Kornegay, 1995. Phytates: Occurrence, bioavailability and implications in poultry nutrition. Poult. Avian Biol. Rev., 6: 125-143.
Direct Link - Sebastian, S., S.P. Touchburn and E.R. Chavez, 1998. Implications of phytic acid and supplemental microbial phytase in poultry nutrition: A review. World Poult. Sci. J., 54: 27-47.
CrossRefDirect Link - Selle, P.H. and V. Ravindran, 2007. Microbial phytase in poultry nutrition. Anim. Feed Sci. Technol., 135: 1-41.
CrossRefDirect Link - Simons, P.C.M., H.A.J. Versteegh, A.W. Jongbloed, P.A. Kemme and P. Slump et al., 1990. Improvement of phosphorus availability by microbial phytase in broilers and pigs. Br. J. Nutr., 64: 525-540.
CrossRefDirect Link - Waldroup, P.W., 1999. Nutritional approaches to reducing phosphorus excretion by poultry. Poult. Sci., 78: 683-691.
CrossRefDirect Link - Waldroup, P.W., J.H. Kersey, E.A. Saleh, C.A. Fritts and F. Yan et al., 2000. Nonphytate phosphorus requirement and phosphorus excretion of broiler chicks fed diets composed of normal or high available phosphate corn with and without microbial phytase. Poult. Sci., 79: 1451-1459.
CrossRefPubMedDirect Link - Yan, F., J.H. Kersey and P.W. Waldroup, 2001. Phosphorus requirements of broiler chicks three to six weeks of age as influenced by phytase supplementation. Poult. Sci., 80: 455-459.
PubMedDirect Link