A total of 30 strains of Escherichia coli O157:H7 isolated from beef and chicken burger were characterized by Enterobacterial Repetitive Intragenic Consensus (ERIC) genotyping. The ERIC polymorphism patterns obtained as illustrated in a dendrogram showed a significant discriminatory fingerprint among the 30 E. coli O157:H7 strains. Nearly every isolates had a unique fingerprint and that there were no bands that were highly conserved among the isolates. This study suggests that there is considerable genetic heterogeneity among the E. coli O157:H7 strains by ERIC PCR, and that this has application in screening strains from clinical or food samples to detect a virulent strain with a known fingerprint, and to trace its dissemination.
How to cite this article
Ooi Wai Ling, Son Radu, Gulam Rusul, Mohamed Ismail Abdul Karim, Endang Purwati and Samuel Lihan, 2000. Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli O157:H7. Pakistan Journal of Biological Sciences, 3: 35-37.