T. Saravanan
Agricultural Research Station, Kovilpatti-628501, India
M. Muthusamy
Agricultural Research Station, Kovilpatti-628501, India
T. Marimuthu
Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore-641003, India
ABSTRACT
A detailed study was conducted to find out the efficacy of Pseudomonas fluorescens on fusarium wilt pathogen in banana rhizosphere. All the strains of P. fluorescens isolated from banana rhizosphere had significant inhibitory action on the growth of Fusarium oxysporum f.sp. cubense. Among the strains, Pfm of P. fluorescens had higher inhibitory action while comparing with other strain. Talc formulation of Pfm of P. fluorescens was prepared and applied in three month old banana plants to study their efficacy on fusarium wilt disease. After three month of inoculation, Pfm of P. fluorescens inoculated at 10 and 15 g plant ha-1 showed lesser vascular discoloration index than the control. To study the rhizosphere colonizing ability in banana rhizosphere against fusarium wilt disease, a rifampicin resistant strain of Pfm of P. fluorescens was developed. The banana plants inoculated with a rifampicin resistant strain of Pfm of P. fluorescens showed gradual increase in population of P. fluorescens in the rhizosphere over the period of time upto 60 days after inoculation. The extracts of soil inoculated with rifampicin resistant strain of Pfm of P. fluorescens at 10 g plant ha-1 showed significant reduction in spore germination of F. oxysporum f.sp. cubense. Gradual increasing in the reduction of spore germination was observed in the soil extracts over the period of inoculation upto 60 days after inoculation. Similarly, the filter paper dipped in extracts of soil had higher inhibition of growth of F. oxysporum f.sp. cubense at 60 days after inoculation.
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How to cite this article
T. Saravanan, M. Muthusamy and T. Marimuthu, 2004. Effect of Pseudomonas fluorescens on Fusarium Wilt Pathogen in Banana Rhizosphere. Journal of Biological Sciences, 4: 192-198.
DOI: 10.3923/jbs.2004.192.198
URL: https://scialert.net/abstract/?doi=jbs.2004.192.198
DOI: 10.3923/jbs.2004.192.198
URL: https://scialert.net/abstract/?doi=jbs.2004.192.198
REFERENCES
- Kloepper, J.W., J. Leong, M. Teintze and M.N. Schroth, 1980. Enhanced plant growth by siderophores produced by plant growth-promoting rhizobacteria. Nature, 286: 885-886.
CrossRefDirect Link - Snyder, W.C. and H.W. Hansen, 1940. The species concept in Fusarium. Am. J. Bot., 27: 64-67.
Direct Link - King, E.O., M.K. Ward and D.E. Raney, 1954. Two simple media for the demonstration of pyocyanin and fluorescin. Transl. Res., 44: 301-307.
PubMedDirect Link - Vidhyasekaran, P. and M. Muthamilan, 1995. Development of formulations of Pseudomonas fluorescens for control of chickpea wilt. Plant Dis., 79: 782-786.
Direct Link - Bonsall, R.F., D.M. Weller and L.S. Thomashow, 1997. Quantification of 2,4-diacetyl phloroglucinol produced by fluorescent Pseudomonas spp. in vitro and in the rhizosphere of wheat. Applied Environ. Microbiol., 63: 951-955.
PubMedDirect Link - Mew, T.W. and A.M. Rosales, 1986. Bacterization of rice plants for control of sheath blight caused by Rhizoctonia solani. Phytopatholgy, 76: 1260-1264.
CrossRef - Kloepper, J.W., N.M. Schroth and T.D. Miller, 1980. Effects of rhizosphere colonization by plant growth-promoting rhizobacteria on potato plant development and yield. Phytopathology, 70: 1078-1082.
CrossRefDirect Link - Keel, C., U. Schnider, M. Maurhofer, C. Voisard and K. Laville et al., 1992. Suppression of root diseases by Pseudomonas fluorescens CHA0: Importance of the bacterial secondary metabolite 2,4-diacetylphloroglucinol. Mol. Plant Microb. Interact., 5: 4-13.
Direct Link - Rosales, A.M., L. Thomashow, R.J. Cook and T.W. Mew, 1995. Isolation and identification of antifungal metabolites produced by rice-associated antagonistic Pseudomonas spp. Phytopathology, 85: 1028-1032.
CrossRefDirect Link - Maurhoffer, M., C. Hase, J.P. Matraux and G. Defago, 1994. Induction of systemic resistance of tobacco to tobacco necrosis virus by the root-colonizing Pseudomonas fluorescens strains CHAO: Influence of the gacA gene and pyoverdine production. Phytopathology, 84: 139-146.
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daniel dembe Reply
Its agood publication in this era of minimising pesticide use.
I would like to get a copy