B. Felfoldi
Department of Basic Sciences,
College of Veterinary Medicine,
Mississippi State University,
Mississippi State, MS 39762,
USA
G.T. Pharr
Department of Basic Sciences,
College of Veterinary Medicine,
Mississippi State University,
Mississippi State, MS 39762,
USA
L.M. Pinchuk
Department of Basic Sciences,
College of Veterinary Medicine,
Mississippi State University,
Mississippi State, MS 39762,
USA
A.M. Cooksey
Department of Basic Sciences,
College of Veterinary Medicine,
Mississippi State University,
Mississippi State, MS 39762,
USA
J.P. Thaxton
Department of Pultry Science. Mississipp State University, Mississipp State, MS 39762, USA
ABSTRACT
The bursa of Fabricius serves as a primary lymphoid organ for the development of a diverse repertoire of B-cells. The embryonic bursa is colonized during embryonic days (ED) 8-14 by stem cells expressing the sialyl Lewisx carbohydrate (SLEX). At ED13-15, cells with the SLEX phenotype initiate proliferation leading to the development of the bursal follicle. By ED15-17, a key differentiation event occurs resulting in the onset of repertoire development by immunoglobulin gene-conversion. This differentiation event is defined by a phenotypic transition in cell surface glycosylation from SLEX to a related carbohydrate structure termed Lewisx(LEX). The goal of this study was to identify functional groups of genes in the two stages that might be involved in critical biological processes of proliferation, differentiation, apoptosis and cell adhesion, and explain the observed functional differences. We found that prior to the onset of immunoglobulin gene conversion B-cells express genes related to ephrin receptor signaling, epidermal growth factor receptor (EGFR) signaling and Wnt signaling. B-cells undergoing immunoglobulin gene conversion express genes from TNFR signaling and both stages were found to express members of Wnt signaling, integrin signaling and EGFR signaling pathways. The differentially expressed pathways agree with previous observations, offering explanation to signals leading to proliferation, differentiation and apoptosis in the two B-cell stages.
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How to cite this article
B. Felfoldi, G.T. Pharr, L.M. Pinchuk, A.M. Cooksey and J.P. Thaxton, 2008. Preliminary Protein Profile Analysis of the Late Embryonic B-Cell Stages in the Chicken Bursa of Fabricius . International Journal of Poultry Science, 7: 117-124.
DOI: 10.3923/ijps.2008.117.124
URL: https://scialert.net/abstract/?doi=ijps.2008.117.124
DOI: 10.3923/ijps.2008.117.124
URL: https://scialert.net/abstract/?doi=ijps.2008.117.124