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Research Article

Effects of Oral Administration of Water Extract of Nigella sativa on the Hypothalamus Pituitary Adrenal Axis in Experimental Diabetes

Kamal M.S. Mansi
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The present study was designed to evaluate the role of water extract of Nigella sativa on the hypothalamus-pituitary-adrenal axis in alloxan-induced diabetic rats. Forty male white rats were divided into four experimental groups control, diabetic. N. sativa-treated and N. sativa-treated diabetic. At the end of the experimental period (3 weeks), animals in all three groups were fasted for 12 h and blood samples were taken for the determination of glucose levels, serum concentrations of insulin, glucagon, corticosterone and Adrenocorticotropic hormone (ACTH ) in four groups. It was found that water extract of Nigella sativa was investigated for hypoglycemic effect in diabetic rats and induced significant reduction in serum glucose from (19.83±1.25 Mmol L-1) in diabetic group to (9.7±1.10 Mmol L-1) in N. sativa- treated diabetic group. However the blood glucose still higher than the control and N. sativa- treated group, serum insulin increased from (0.54±0.22 Mu L-1) in control group to (0.65±0.06 Mu L-1) in N. sativa –treated group and still higher than control in N. sativa –treated diabetic (0.58±0,06 Mu L-1), serum corticosterone increased in diabetic group (580 ± 22.36 nmol L-1 )compared to control group (311±18.42 nmol L-1) and decreased in N. sativa-treated (238±16.53 nmol L-1) and in N. sativa treated diabetic group (378±19.65 nmol L-1) and still higher than control. Serum Adrenocorticotropic hormone (ACTH) increased in diabetic group (20.72±2.42 pmol L-1 ) compared to control group (13.82±1.83 pmol L-1) and still lower in N. sativa -treated (10.64±13 pmol L-1) and in N. sativa treated diabetic group (15.42±1.18 pmol L-1) compared to diabetic group. The results suggest the beneficial role of N. sativa as hypoglycemic agents and as a protective effect against pancreatic ß-cells damage from alloxan induced diabetes in rats by decreasing oxidative stress and preserving pancreatic ß -cells integrity and also suggest that the antidiabetic effect of N. sativa may be attributed to increased glucose metabolism by increasing the serum concentration of insulin and inhibited the hypothalamus-pituitary-adrenal axis.

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  How to cite this article:

Kamal M.S. Mansi , 2006. Effects of Oral Administration of Water Extract of Nigella sativa on the Hypothalamus Pituitary Adrenal Axis in Experimental Diabetes. International Journal of Pharmacology, 2: 104-109.

DOI: 10.3923/ijp.2006.104.109


1:  Tich, R. and H. McDevit, 1996. Insulin dependent diabetes mellitus. Cell, 85: 291-297.

2:  Bell, G.I. and K.S. Polonsky, 2001. Diabetes mellitus and genetically programmed defects in β-cell function. Nature, 414: 788-791.
Direct Link  |  

3:  Zimmet, P., K.G.M.M. Alberti and J. Shaw, 2001. Global and societal implications of the diabetes epidemic. Nature, 414: 782-787.
CrossRef  |  PubMed  |  Direct Link  |  

4:  Klip, A., A. Marette, D. Dimitrakouids, T. Ramlal and M. Shizq Varnic, 1992. Effect of diabetes on glucoregulation. From glucose transports to glucose metabolism in vivo. Diabetes Care, 15: 1747-1766.
Direct Link  |  

5:  Taskinen, M.R., S. Lahdenpera and M. Syvanne, 1996. New insights into lipid metabolism in non-insulin-dependent diabetes mellitus. Ann. Med., 28: 335-340.
Direct Link  |  

6:  Vlassara, H., M. Brownlee and A. Cerami, 1984. Accumulation of diabetic rat peripheral nerve myelin by macrophages increases with the presence of advanced glycosylation end products. J. Exp. Med., 160: 197-207.

7:  Yabe-Nishimura, C., 1998. Aldose reductase in glucose toxicity: A potential target for the prevention of diabetic complication. Pharmacol. Rev., 50: 21-34.
Direct Link  |  

8:  Brownlee, M., 2001. Biochemistry and molecular cell biology of diabetic complications. Nature, 414: 813-820.
CrossRef  |  PubMed  |  Direct Link  |  

9:  Saks, D.B., 1997. Implication of the revised criteria for diagnosis and classification of diabetes mellitus. Clin. Chem., 43: 2230-2232.
Direct Link  |  

10:  Palmieri, V., J.N. Bella, D.K. Arnett, J.E. Liu and A. Oberman et al., 2001. Effect of type 2 diabetes mellitus on left ventricular geometry and systolic function in hypertensive subjects: Hypertension genetic Epidemiology network Hyper GEN Study. Circulation, 103: 102-107.
CrossRef  |  PubMed  |  Direct Link  |  

11:  Yenigum, M., 1997. Cardiovascular diabetes. Istanbul University printing house, Istanbul, Turkey, 43: 2230-2233.

12:  Garcia Leme, J., L. Hamamura, R.H. Migliorini and M.P. Leite, 1973. Experimental diabetes and inflammatory reactions in the rat. Agents Actions, 3: 380-381.
CrossRef  |  Direct Link  |  

13:  Garcia Leme, J. and S.P. Farsky, 1993. Hormonal control of inflam- matory response. Mediators of Inflammation, 2: 181-198.
CrossRef  |  Direct Link  |  

14:  Rayfield, E.J., M.J. Ault, G.T. Keusch, M.J. Brothers, C. Nechemias and H. Smith, 1982. Infection and diabetes: The case for glucose control. Am. J. Med., 72: 439-450.
Direct Link  |  

15:  Weitzman, E.D., D. Fukushima, C. Nogeire, H. Roffwarg, T.F. Gallagher and L. Hellman, 1971. Twenty-four-hour pattern of the episodic secretion of cortisol in normal subjects. J. Clin. Endocrinol. Metab., 33: 14-22.
Direct Link  |  

16:  Cameron, O.G., Z. Kronfol, J.F. Grenden and B.J. Carroll, 1984. Hypothalamic-pituitary-adrenocortical activity in patients with diabetes mellitus. Arch. Gen. Psychiatry, 41: 1090-1095.
PubMed  |  

17:  Coiro, V., R. Volpi, L. Capretti, G. Speroni and P. Caffarra et al., 1995. Low-dose corticotrophin-releasing hormone stimulation test in diabetes mellitus with or without neuropathy. Metabolism, 44: 538-542.
Direct Link  |  

18:  Roy, M., B. Collier and A. Roy, 1990. Hypothalamic-pituitary-adrenal axis dysregulation among diabetic outpatients. Psychiatry Res., 31: 31-37.
Direct Link  |  

19:  Roy, M.S., A. Roy and W.T. Gallucci, 1993. The ovine corticotropin-releasing hormone-stimulation test in type I diabetic patients and controls: Suggestion of mild chronic hypercortisolism. Metabolism, 42: 696-700.
Direct Link  |  

20:  Fehm, H.L., R. Holl, E. Spath-Schwalbe, J. Born and K.H. Voigt, 1988. Ability of corticotropin releasing hormone to stimulate cortisol secretion independent from pituitary adrenocorticotropin. Life Sci., 42: 679-686.

21:  Hudson, J.I., M.S. Hudson, A.J. Rothschild, L. Vignati, A.F. Schatzberg and J.C. Melby, 1989. Abnormal results of dexamethasone suppression tests in non-depressed patients with diabetes mellitus. Arch. Gen. Psychiatry, 41: 1086-1089.

22:  Sapolsky, R.M., 1996. Glucocorticoids and damage to the nervous system: The current state of confusion. Stress, 1: 1-19.
Direct Link  |  

23:  Kadekaro, M., M. Ito and P.M.Gross, 1988. Local cerebral glucose utilization is increased in acutely adrenalectomized rats. Neuroendocrinology, 47: 329-334.

24:  Doyle, P., F. Rohner-Jeanrenaud and B. Jeanrenaud, 1993. Local cerebral glucose utilization in brains of lean and genetically obese (fa/fa) rats. Am. J. Physiol., 264: E29-E36.
Direct Link  |  

25:  Horner, H., D. Packan and R. Sapolsky, 1990. Glucocorticoids inhibit glucose transport in cultured hippocampal neurons and glia. Neuroendocrinol., 52: 57-64.
Direct Link  |  

26:  Reagan, L.P., A.M. Magarinos and B.S. McAwen, 1999. Neurological changes induced by stress in streptozotocin diabetic rats. Ann. New York Acad. Sci., 893: 126-137.
Direct Link  |  

27:  McCall, A.L., 1992. The impact of diabetes on CNS. Diabetes, 41: 557-570.
Direct Link  |  

28:  Ahmad, F., P.M.M. Khalid, M. Khan, A.K. Chaubey, Rastogi and J.R. Kidwai, 1995. Hypoglycemic activity of pterocarpus marsupium wood. J. Ethenopharm., 35: 71-75.

29:  Khosia, P., D. Gupts and R.K. Nagpal, 1995. Effect of trigonella foenom graecum fenugreet on blood glucose in normal and diabetic rats. Ind. J. Physiol. Pharmacol., 39: 173-174.

30:  Rai, V., U. Lyer and U.V. Mani, 1997. Effect of Tulasi Ocimum sanctum leaf powder supplementation on blood sugar levels serum lipids and tissue lipids in diabetic rats. Plant Foods Human Nut., 50: 9-16.
PubMed  |  Direct Link  |  

31:  Manisckam, M.M., M.A. Ramanthan, J.P. Jahromi, J. Chasouria and A.B. Ray, 1997. Antihyperglycemic activity of phenolics from Pterocapus marsupium. J. Nat. Products, 60: 609-610.
CrossRef  |  Direct Link  |  

32:  Zubaida, A., A. Basil, A. Abdullah and Bomosa, 2001. Effect of Nigella sativa (Black seed) and thymoquin on blood glucose in albino rats Ann. Saudi Med., 21: 18-27.

33:  Agel, M. and R. Shaheen, 1996. Effects of the volatile oil of Nigella sativa seeds on the uterine smooth muscle of rat and guinea pig. J. Ethnopharmacol., 52: 23-26.
CrossRef  |  Direct Link  |  

34:  El Tahir, K.E.H., M.M.S. Ashour and M.M. Al-Harbi, 1993. The Cardiovascular actions of the volatile oil of the black seed (Nigella sativa) in rats: Elucidation of the mechanisms of action. Gen. Pharmar., 24: 1123-1131.
CrossRef  |  

35:  Zaoui, A., Y. Cherrah, M.A. Lacaille-Dubois, A. Settaf, H. Amarouch and M. Hassar, 2000. Diuretic and hypotensive effects of Nigella sativa in the spontaneously hypertensive rat. Therapie, 55: 379-382, (In French).
PubMed  |  Direct Link  |  

36:  Tuk, J., 2003. Effect of Nigella sativa on liver necrosis. Vet. Anim. Sci., 27: 141-152.

37:  Abdel, M.A., M. El Feki and E. Saleh, 1998. Effect of Nigella sativa, fish oil and localized on alloxan diabetic rats. Bioch. histopath studies. J. Egypt. Ger. Soc. Zool., 23: 237-265.

38:  Ismail, M., Y. Ozbek and R. Ustun, 2003. Effects of Nigella sativa on serum concentration TSH and glucose in induced diabetic rabbits. J. Irish Vet., 56: 446-484.

39:  Al-Awadi, F.M. and K.A. Gumaa, 1987. Studies on the activity of individual plants of an antidiabetic plant mixture. Acta Diabetol., 24: 37-41.
CrossRef  |  Direct Link  |  

40:  Shapiro, E.T., K.S. Polonsky, G. Copinschi, D. Bosson, H. Tillil, J. Blackman, G. Lewis and E. Van Cauter, 1991. Nocturnal elevation of glucose levels during fasting in noninsulin-dependent diabetes. J. Clin. Endocrinol. Metabol., 72: 444-454.
Direct Link  |  

41:  Scribner, K.A., S.F Akana, C.D. Walker and M.F. Dolman, 1993. Streptozotocin-diabetic rats exhibit facilitated adrenocorticotropin responses to acute stress, but normal sensitivity to feedback by corticosteroids. Endocrinology, 133: 2667-2674.

42:  De Nicola, A.F., O. Fridman, E.J. Del Castillo and V.G. Foglia, 1976. The influence of streptozotocin diabetes on adrenal function in male rats. Horm. Metabol. Res., 8: 388-392.

43:  Chan, O., S. Chan, K. Iouye, M. Vrnic and S.G. Matthews, 2001. Molecular regulation of the Hypothalamus-Pituitary-Adrenal (HPA) axis in streptozotocin-induced diabetes: Effects of insulin treatment. Endocrinology, 142: 4872-4879.
Direct Link  |  

44:  Unger, R.H., 1978. Role of glucagon in the pathogenesis of diabetes: The status of the controversy. Metabolism., 27: 1691-1709.

45:  Lefebvre, P.J.and A.S. Luyckx, 1979. Glucagon and diabetes: A reappraisal. Diabetologia., 16: 347-354.
Direct Link  |  

46:  Reagan, L.P., A.M. Magarinos, L.R. Lucas, A. Van Bueren, A.L. McCall and B.S. McEwen, 1999. Regulation of GLUT-3 glucose transporter in the hippocampus of diabetic rats subjected to stress. Am. J. Physiol., 276: E879-E886.
Direct Link  |  

47:  Cryer, P.E. and J.E. Gerich, 1997. Hypoglycemia in Insulin-Dependent Diabetes Mellitus: Interplay of Insulin Excess and Comprised Glucose Counterregulation. In: Ellenberg and Rifkin's Diabetes Mellitus, Porte, Jr. D. and R.S. Sherwin (Eds.). Appleton and Lange, Stamford, CT, pp: 745-760.

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