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Pharmacological Characterization of the Rat` s Paw Oedema Induced by Echis coloratus Venom



Abdulrahman Khazim Al Asmari
 
ABSTRACT

The present study was conducted to investigate the inflammatory response induced by Echis coloratus venom (ECLV) in the rat hind-paw by measuring paw oedema. Non-heated ECLV (50 μg paw -1) caused a marked paw oedema (plateau) accompanied by intense haemorrhage whereas heated venom (97 °C, 30 s; 3.125-75 μ g paw -1) produced a dose and time-dependent non-haemorrhagic oedema. The response with heated ECLV was maximal within 15 min, disappearing over 24 h. Heated ECLV (50 μ g paw -1) was optimized to test the effect of various drugs on oedema induced by the ECLV. The results showed that cyproheptadine (H1 and H3) receptor antagonist highly (P < 0.001) reduced venom-induced rat paw oedema and was moderately (P<0.01) reduced by dexamethasone. A proteinase inhibator (aprotinin), cyclooxygenase inhibitor (indomethacin) and histamine (H1) receptor antagonist (chlorpheniramine) produced a low but significant inhibition of oedema formation. The commercially available antivenom was found to be ineffective when administered intravenously, whereas its local administration partially reduced rat paw oedema induced by ECLV, but was not statistically significant. The present study concluded that ECLV alone induced oedema and the expected principal mediators of this inflammatory response were serotonin, histamine, cyclo-oxygenase and other prostaglandins (PGs) and cytokines. Finally the polyspecific antivenom given intravenously could not prevent the oedema forming effect in rats.

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  How to cite this article:

Abdulrahman Khazim Al Asmari , 2003. Pharmacological Characterization of the Rat` s Paw Oedema Induced by Echis coloratus Venom. Journal of Biological Sciences, 3: 309-319.

DOI: 10.3923/jbs.2003.309.319

URL: https://scialert.net/abstract/?doi=jbs.2003.309.319

REFERENCES
Abdel-Nabi, I., R. Awadalla and I. El-Shamy, 1997. Biochemical effects of intraperitoneal injection of rats with the venom of the snake Echis carinatus. Egypt. J. Zool., 29: 195-205.

Abu-Sinna, G., A.S. Al-Zahaby, A.A. El-Aal, A.A. El-Baset and N.A. Soliman, 1993. The effect of the viper Cerastes cerastes cerastes venom and venom fractions on carbohydrate metabolism. Toxicon, 31: 791-801.
CrossRef  |  Direct Link  |  

Al-Jammaz, I., M.K. Al-Sadoon and A. Faheem, 1999. Effect of LD50 Dose of Echis coloratus venom on serum and tissue metabolites and some enzymes of male albino rats. J. King Saud Univ. Sci., 11: 61-68.
Direct Link  |  

Al-Jammaz, I., M.K. Al-Sadoon and A. Faheem, 1999. Effect of LD50 Dose of Echis coloratus venom on serum and tissue metabolites and some enzymes of male albino rats. J. King Saud Univ. Sci., 11: 61-68.
Direct Link  |  

Al-Sadoon, M.K. and S.A. Al-Farraj, 1992. Venomous Snakes of Saudi Arabia: Species, Distribution and Precautionary Methods. Al-Resalah Press, Riyadh, Saudi Arabia, pp: 107.

Al-Sadoon, M.K., S.A. Al-Farraj and N.M. Abdo, 1991. Survey of the reptilian fauna of the Kingdom of Saudi Arabia. III. An ecological survey of the lizard, amphisbaenian and snake fauna of Al-Zulfi Area. Maryland Herpetol. Soc., 27: 1-22.

Angeli, A., R.G. Masera, M.L. Sartori, N. Fortunati and S. Racca et al., 1999. Modulation by cytokines of glucocorticoid action. Ann. N. Y. Acad. Sci., 876: 210-220.
Direct Link  |  

Assakura, M.T., A.P. Reichl and F.R. Mandelbaum, 1986. Comparison of immunological biochemical and biophysical properties of three hemorrhagic factors isolated from the venom of Bothrops jararaca (jararaca). Toxicon., 24: 943-946.
Direct Link  |  

Benbassat, J. and O. Shalev, 1993. Envenomation by Echis coloratus (Mid-East saw-scaled viper): A review of the literature and indications for treatment. Israel J. Med. Sci., 29: 239-250.
PubMed  |  Direct Link  |  

Bertke, E.M. and J.H. Atkins, 1961. Effect of Centruroides sculpturatus venom upon rat tissue: A histopathologic study. Toxicon, 2: 205-209.
CrossRef  |  Direct Link  |  

Bjarnason, J.B. and J.W. Fox, 1994. Hemorrhagic metalloproteinases from snake venoms. Pharmacol. Ther., 62: 325-372.
Direct Link  |  

Brain, S.D. and T.J. Williams, 1985. Inflammatory mechanism of inflamed-tissue factor. Agents Actions, 3: 348-356.

Buckley, T.L., S.D. Brain, P.D. Collins and T.J. William, 1991. Inflammation oedema induced by interactions between interleukin-1 and neuropeptide calcitonin gene-related peptide. J. Immunol., 146: 3424-3430.

Cherlin, V.A. and L.J. Brokin, 1990. Taxonomic revision of the snake genus Echis (Viperidae). II An analysis of the history of study and synonymy. USSR Acad. Sci. Proc. Zool. Inst. Leningrad, 207: 175-192.

Erdos, E.G., 1963. Structure and function of biologicaly active peptides: Bradykinin, kallidin and congeners. Ann. N. Y. Acad. Sci., 104: 91-107.

Faria, L., E. de Antunes, C. Bon and A. de Lobo, 2001. Pharmacological characterisation of the rat paw oedema induced by Bothrops lanceolatus (Fer de Lance) venom. Toxicon, 39: 825-830.

Flower. R.J., 1989. Glucocorticoids and the Inhibition of Phospholipase A2. In: Antiinflammatory Steroid Action Basic and Clinic Aspects, Schlelmer, R.P., H.N. Clamor and A.L. Oronsky (Eds.). Academic Press, New York, pp: 48-66.

Goth, A., 1978. Medical Pharmacology. C.V. Mosby Company, St. Louis, Missouri, pp: 766.

Gutierrez, J.M., F. Chaves and L. Cerdas, 1986. Inflammatory infiltrate in skeletal muscle injected with Bothrops asper venom. Rev. Biol. Trop., 34: 209-219.
Direct Link  |  

Hamblin, A.S., 1994. Cytokines. In: Textbook of Immunopharmacology, Dale, M., J.C. Foreman and D.F.T. Ping (Eds.). 3rd Edn., Blackwell Scientific Publications, London, pp: 179-192.

Issekutz, A.C., 1981. Effect of vasoactive agents on polymorphonuclear leukocyte emigration in vivo. Lab. Invest., 45: 234-240.
Direct Link  |  

Johnson, A.R. and E.G. Erdos, 1973. Release of histamine from mast cells by vasoactive peptides. Proc. Soc. Exp. Biol. Med., 142: 1252-1256.
Direct Link  |  

Jorge, M.T., L.A. Ribeiro and J.L. OConnell, 1999. Prognostic factors for amputation in the case of envenoming by snakes of the Bothrops genus (Viperidae). Ann. Trop. Med. Parasitol., 93: 401-408.

Kamiguti, A.S., J.L. Cardoso, R.D. Theakston, I.S. Sano-Martins and R.A. Hutton et al., 1991. Coagulopathy and haemorrhage in human victims of Bothrops jararaca envenoming in Brasil. Toxicon, 9: 961-972.
Direct Link  |  

Kingston, M.E., 1981. Management of snake bite in Saudi Arabia. J. King Faisal Specialist Hospital Med., 1: 87-94.

Lobo de Araujo, A., J.L. Donato and C. Bon, 1998. Purification from Bothrops lanceolatus (Fer de lance) venom of a fibrino(geno)lytic enzyme with esterolytic activity. Toxicon, 36: 745-758.
Direct Link  |  

Lobo-de-Araujo, A., A.O. de Souza, M.A. da Cruz-Hofling, C.A. Flores and C. Bon, 2000. Bothrops lanceolatus (Fer de lance) venom induces oedema formation and increases vascular permeability in the mouse hind paw. Toxicon, 38: 209-221.
Direct Link  |  

Lobo-de-Araujo, A., F. Radvanyi and C. Bon, 1994. Acidic phospholipase A2 from Bothrops lanceolatus venom: purification and molecular, enzymatic and pharmacological properties. Toxicon, 32: 1069-1081.
PubMed  |  Direct Link  |  

Lomonte, B., A. Tarkowski and L.A. Hanson, 1993. Host response to Bothrops asper snake venom. Analysis of oedema formation, inflammatory cells and cytokine release in a mouse model. Inflammation, 17: 93-105.
Direct Link  |  

Mahmoud, I., 1983. Biochemical and physiological studies on the action of viper venom Cerastes vipera on mice Mus musculus. Masters Thesis, Fac. Sci. Cairo University, Egypt.

Mandelbaun, F.R., A.P. Reichl and M.T. Assakura, 1975. Some Physical and Biochemical Characteristics of HF2 one of the Hemorrhagic Factors in the Venom Bothrops Jararaca. In: Animal Plant and Microbial Toxins, Ohsaka, A., K. Hayashi and Y. Sawai (Eds.). Plenum Press, New York, Tokyo, pp: 111-121.

Marsh, N., D. Gattullo, P. Pagliaro and G. Losano, 1997. The gaboon viper, Bitis gabonica: Haemorrhagic, metablic, cardiovascular and clinical effects of the venom. Life Sci., 61: 763-769.

Milani-Junior, R., M.T. Jorge, F.P. de Campos, F.P. Martins and A. Bousso et al., 1997. Snake bites by jararacucu (Othrops jararacussu): Clinicopathological studies of 29 proven cases in Sao Paulo State. Brasil. QJM, 90: 323-334.
Direct Link  |  

Moav, B., C. Moroz and A. de Vries, 1963. Activation of the fibrinolytic system of the guinea pig following inoculation of Echis colorata venom. Toxicon, 1: 109-112.

Mohamed, A.H. and L.Z. Khaled, 1966. Effect of the venom of Cerastes cerastes on nerve tissue and skeletal muscle. Toxicon, 3: 223-224.
CrossRef  |  PubMed  |  Direct Link  |  

Moustafa, F.A., Y.Y. Ahmed and M.F. El-Asmer, 1974. Effect of scorpion Buthus minax L. Koch venom on succinic dehydrogenase and cholinesterase activity of mouse tissue. Toxicon, 12: 237-240.

Perales, J., C.Z. Amorim, S.L.G. Rocha, G.B. Domont and H. Moussatche, 1992. Neutralization of the oedematogenic activity of Bothrops jararaca venom on the mouse paw by an antibothropic fraction isolated from opossum (Didelphis marsupialis) serum. Agents Act., 37: 250-259.
CrossRef  |  

Rosenfeld, G., 1971. Symptomatology Pathology and Treatment of Snake Bites in South America. In: Venomous Animals and their Venoms, Bucherl, W. and E.E. Buckley (Eds.). Acad. Press, New York, pp: 345-403.

Schaeffer, R.C., C. Briston, S.M. Chilton and R.W. Carlson, 1986. Disseminated intravascular coagulation following Echis carinatus venom in dogs: Effects of a synthetic thrombin inhibitor. J. Lab. Clin. Med., 107: 488-497.

Tan, N.H. and G. Ponnudurai, 1990. A comparative study of the biological properties of venoms from snakes of the genus Vipera (true adders). Comp. Biochem. Physiol. B, 96: 683-688.
Direct Link  |  

Theakston, R.D.G., 1983. The application of immunoassay techniques including enzyme-linked immunoassay (ELISA) to snake venom research. Toxicon, 21: 341-352.
Direct Link  |  

Theakston, R.D.G., H.A. Reid and D. Iddon, 1982. Standardization tests for estimation of defibrinating, coagulant, haemorrhagic and necrotizing effects of snake venom. Toxicon, 20: 363-363.

Tilbury, C.R., M.M. Madkour, D. Saltissi and M. Suleiman, 1987. Acute renal failure following the bite of Burton's carpet viper Echis coloratus Gunther in Saudi Arabia: Case report and review. Saudi Med. J., 8: 87-95.
Direct Link  |  

Warrell, D.A. and C. Arnett, 1976. The importance of bites by the saw-scaled or carpet viper (Echis carinatus): Epidemiological studies in Nigeria and a review of the world literature. Acta Tropica, 33: 307-341.
Direct Link  |  

Warrell, D.A. and C. Arnett, 1976. The importance of bites by the saw-scaled or carpet viper (Echis carinatus): Epidemiological studies in Nigeria and a review of the world literature. Acta Tropica, 33: 307-341.
Direct Link  |  

Warrell, D.A., 1995. Clinical Toxicology of Snake Bite in Africa and the Middle East/Arabian Peninsula. In: Handbook of Clinical Toxicology of Animal Venoms and Poisons, Meier, J. and J. White (Eds.). CRC Press, Boca Raton, Florida, pp: 433-492.

Warrell, D.A., N.M.D. Davidson and B.M. Greenwood, 1977. Poisoning by bites of the saw-scaled or carpet viper (Echis carinatus) in Nigeria. Q. J. Med., 46: 33-62.
Direct Link  |  

Weiss, H.J., L.L. Phillips, W.S. Hopewell, G. Phillips, N.P. Christy and R.F. Nitti, 1973. Heparin therapy in a patient bitten by a saw scaled viper (Echis carinatus), a snake whose venom activates prothrombin. Am. J. Med., 54: 653-662.

Williams, T.J. and J. Morley, 1973. Prostaglandins as potentiators of increased vascular permeability in inflammation. Nature, 246: 215-217.
CrossRef  |  Direct Link  |  

Williams, T.J. and M.J. Peck, 1977. Role of prostaglandin-mediated vasodilataion in inflammation. Nature, 270: 530-532.
Direct Link  |  

Williams, T.J., 1979. Prostaglandin E2, prostaglandin I2 and the vascular changes in inflammation. Br. J. Pharmacol., 65: 517-524.
Direct Link  |  

Williams, T.J., 1983. Interactions between prostaglandins, leukotrienes and other mediators of inflammation. Br. Med. Bull., 39: 239-242.
Direct Link  |  

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