Narayan Roy
Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh
Md. Zainul Abedin
Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh
ABSTRACT
The xylanase-producing bacterial strains were isolated from drain water. The strains were isolated on xylan-agar media and screening was carried out by β-xylanolysis method. The strains were further confirmed by DNS-method. The isolated strains were identified as Aeromonas on the basis of catalase, oxidase, gram staining and morphological cultural characteristics as well as by different biochemical tests. Multi-drug resistant of these strains were confirmed by antibiotic spread plate method. Disc diffusion method and antibiotic spread plate method showed the same multi-drug resistant pattern. Plasmid DNA was isolated from maximum xylanase-producing strain. Agarose gel electrophoresis of the isolated plasmid DNA in 0.8% agarose showed the presence of only one 30 kb plasmid. The plasmid DNA was transformed into nonxylanase-producing sensitive E. coli LE392. The transformants showed xylanase activity as well as multi-drug resistance. Xylanase activity and multi-drug resistance in Aeromonas strain was proposed to be plasmid mediated.
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How to cite this article
Narayan Roy and Md. Zainul Abedin, 2002. Isolation and Molecular Characterization of Xylanase-Producing Bacteria from Drain Water. Journal of Biological Sciences, 2: 399-402.
DOI: 10.3923/jbs.2002.399.402
URL: https://scialert.net/abstract/?doi=jbs.2002.399.402
DOI: 10.3923/jbs.2002.399.402
URL: https://scialert.net/abstract/?doi=jbs.2002.399.402
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