Ikram -ul-Haq
Biotechnology Research laboratory, Department of Botany, Government College, Lahore, Pakistan
Ayesha Khan
Biotechnology Research laboratory, Department of Botany, Government College, Lahore, Pakistan
Waseem Ahmad Butt
Biotechnology Research laboratory, Department of Botany, Government College, Lahore, Pakistan
Sikander Ali
Biotechnology Research laboratory, Department of Botany, Government College, Lahore, Pakistan
M. A. Qadeer
Centre of Excellence in Molecular Biology (CEMB), Thakkar Niaz Baig, Lahore, Pakistan
ABSTRACT
The present study is concerned with the effect of carbon and nitrogen sources and their concentrations on the production of xylanase by mutant strain of Aspergillus niger GCBMX-45. High xylanase activities (2350 U/g) were observed when starch (2%) was used as carbon source. (NH4)2SO4 (0.2%) was found to be best nitrogen source for optimum enzyme production i.e., 2480 U/g. The production of enzyme reached maximum, 72 hours after inoculation. All the fermentations were carried out at 30°C at 0.4 cm (10g wheat bran) depth of the basal medium.
PDF References
How to cite this article
Ikram -ul-Haq, Ayesha Khan, Waseem Ahmad Butt, Sikander Ali and M. A. Qadeer, 2002. Effect
of Carbon and Nitrogen Sources on Xylanase Production by Mutant Strain
of Aspergillus niger GCBMX-45. Journal of Biological Sciences, 2: 143-144.
DOI: 10.3923/jbs.2002.143.144
URL: https://scialert.net/abstract/?doi=jbs.2002.143.144
DOI: 10.3923/jbs.2002.143.144
URL: https://scialert.net/abstract/?doi=jbs.2002.143.144
REFERENCES
- Gawande, P.V. and M.Y. Kamat, 1999. Production of Aspergillus xylanase by lignonocellulosic waste fermentation and its application. J. Applied Microbiol., 87: 511-519.
PubMed - Gokhale, D.V., S.G. Patil and K.B. Bastawde, 1991. Optimization of cellulase production by Aspergillus niger NCIM 1207. Applied Biochem. Biotechnol., 30: 99-109.
CrossRefPubMedDirect Link - Kulkarni, N., A. Shendye and M. Rao, 1999. Molecular and biotechnological aspects of xylanases. FEMS Microbiol. Rev., 23: 411-456.
CrossRef - Miller, G.L., 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 31: 426-428.
CrossRefDirect Link - Shamala, T.R. and K.R. Sreekantiah, 1987. Successive cultivation of selected cellulolytic fungi on rice straw and wheat bran for economic production of cellulases and D-xylanase. Enzyme Microbiol. Technol., 9: 97-101.
CrossRefDirect Link - Someet, N., S. Virendra and V. Bisaria, 2001. Optimization of xylanase production by Melanocarpus albomyces IIS 68 in solid state fermentation using response surface methodology. J. Biosci. Bioeng., 91: 425-427.
Direct Link
shabir ahmad Reply
my work is related with this work so i want full length paper of above can u please send it to me
Muzaffar Reply
You can download full length paper by clicking on "[Full text PDF]"