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Research Article
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Antioxidant and Antiproliferative Effects of Flavonoids from Emilia sonchifolia Linn on Human Cancer Cells |
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T.R. Cibin,
G. Srinivas,
D. Gayathri Devi,
Priya Srinivas,
Y. Lija
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Annie Abraham
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ABSTRACT
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In this study we have looked into a fairly less studied, common edible plant- Emilia sonchifolia Linn (ES), for possible antioxidant and antitumor activity. We have isolated the flavonoid fraction from the whole plant and the antioxidant property was assessed by measuring its capacity to inhibit Cu2+ induced lipoprotein oxidation in human serum and superoxide production in vitro in comparison with Quercetin. The lag time for ES fraction was 60 minutes, whereas for Quercetin it was 72 min. The inhibitory concentration 50% (IC50) values obtained for superoxide production was 48 μg (Flavonoid fraction of ES) compared to 32 μg for Quercetin. Based on these results, the efficiency of the plant product to inhibit the proliferation of human cancer cells were evaluated. Treatment of the cancer cells with the flavonoid fraction of ES showed morphological changes characteristics of apoptosis, confirmed by Ethidium bromide-Acridine Orange staining.
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REFERENCES |
Eskin, N.A.M., E. Hoehn and C. Frenkel, 1978. A simple and rapid quantitative method for total phenols. J. Agric. Food Chem., 26: 973-974. CrossRef | Direct Link |
Gao, J.J., D.L. Cheng and X.P. Liu, 1993. Chemical constituents of Emilia sonchifolia L. DC. Zhongguo Zhong Yao Za Zhi, 18: 102-103. Direct Link |
Garban, H.J. and B. Bonavida, 1999. Nitric oxide sensitizes ovarian tumor cells to Fas-induced apoptosis. Gynecol. Oncol., 73: 257-264. Direct Link |
Gayathri, D.V., P.G. Biju, D.G. Devi, T.R. Cibin, Y. Lija and A. Abraham, 2006. Isolation of Robinetin from Adenanthera pavonina and its protective effects on perchlorate induced oxidative damage. J. Trop. Med. Plants, 7: 111-117.
Hodyson, J.M., J.M. Proudfoot, K.D. Croft, I.B. Puddy, T.A. Mori and L.J. Beelin, 1999. In vitro antioxidant activity of black and green tea: Effects on lipoprotein oxidation in human serum. J. Sci. Food Agric., 79: 561-566. Direct Link |
Hodgson, J.M., I.B. Puddey, K.D. Croft, V. Burke, T.A. Mori, R.A. Caccetta and L.J. Beilin, 2000. Acute effects of ingestion of black and green tea on lipoprotein oxidation. Am. J. Clin. Nutr., 71: 1103-1107. CrossRef | Direct Link |
Muko, K.N. and F.C. Ohiri, 2000. A preliminary study on the anti-inflammatory properties of Emilia sonchifolia leaf extracts. Fitoterapia, 71: 65-68. CrossRef | Direct Link |
Nayar, S.L., R.N. Chopra and I.C. Chopra, 1956. Glossary of Indian Medicinal Plants. Council of Scientific and Industrial Research, New Delhi, India, Pages: 94
Nayar, S.L., R.N. Chopra and I.C. Chopra, 1996. Glossary of Indian Medicinal Plants. National Institute of Science Communication, New Delhi, India
Oommen, S., R.J. Anto, G. Srinivas and G. Karunagaran, 2004. Allicin (from garlic) induces caspase-mediated apoptosis in cancer cells. Eur. J. Pharmacol., 485: 97-103. Direct Link |
Pedraza-Chaverri, J., M. Gil-Ortiz, G. Albarran, L. Barbachano-Esparza, M. Menjivar and O.N. Medina-Campos, 2004. Garlic's ability to prevent in vitro Cu 2+ induced lipoprotein oxidation in human serum is preserved in heated garlic: Effect unrelated to Cu 2+ chelation. Nutr. J., 3: 10-20. Direct Link |
Rastogi, R.P. and B.N. Mehrotra, 1998. Compendium of Indian Medicinal Plants. Central Drug Research Institute Publications and Information Directorate, Lucknow and New Delhi, pp: 3-263
Rastogi, R.P. and B.N. Mehrotra, 1998. Compendium of Indian Medicinal Plants. Vol. 4, Central Drug Research Institute Publications and Information Directorate, Lucknow and New Delhi, pp: 285
Shylesh, B.S. and J. Padikkala, 2000. In vitro cytotoxic and antitumor property of Emilia sonchifolia (L.) DC in mice. J. Ethnopharmacol., 73: 495-500. Direct Link |
Srinivas, G., R.J. Anto, P. Srinivas, S. Vidhyalakshmi, V.P. Senan and D. Karunagaran, 2003. Emodin induces apoptosis of human cervical cancer cells through poly (ADP-ribose) polymerase cleavage and activation of caspase-9. Eur. J. Pharmacol., 473: 117-125. Direct Link |
Srinivas, P., G. Gokul, A. Banerji, A. Dinakar and G. Srinivas, 2004. Plumbagin induces reactive oxygen species, which mediate apoptosis in human cervical cancer cells. Mol. Carcinogenesis, 40: 201-211. Direct Link |
Steel, R.G.D., J.H. Torrie and D.A. Dickie, 1997. Principles and Procedures of Statistics: A Biometrical Approach. 3rd Edn., McGraw-Hill, New York, Pages: 182
Winterbourn, C.C., R.E. Hawkins, M. Brian and R.W. Carrell, 1975. The estimation of red cell superoxide dismutase activity. J. Lab. Clin. Med., 85: 337-341. PubMed | Direct Link |
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