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Research Article
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Antioxidant and Antiproliferative Effects of Flavonoids from Emilia sonchifolia Linn on Human Cancer Cells
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T.R. Cibin ,
G. Srinivas ,
D. Gayathri Devi ,
Priya Srinivas ,
Y. Lija
and
Annie Abraham
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ABSTRACT
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In this study we have looked into a fairly less studied, common edible plant- Emilia sonchifolia Linn (ES), for possible antioxidant and antitumor activity. We have isolated the flavonoid fraction from the whole plant and the antioxidant property was assessed by measuring its capacity to inhibit Cu2+ induced lipoprotein oxidation in human serum and superoxide production in vitro in comparison with Quercetin. The lag time for ES fraction was 60 minutes, whereas for Quercetin it was 72 min. The inhibitory concentration 50% (IC50) values obtained for superoxide production was 48 μg (Flavonoid fraction of ES) compared to 32 μg for Quercetin. Based on these results, the efficiency of the plant product to inhibit the proliferation of human cancer cells were evaluated. Treatment of the cancer cells with the flavonoid fraction of ES showed morphological changes characteristics of apoptosis, confirmed by Ethidium bromide-Acridine Orange staining.
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