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International Journal of Pharmacology

Year: 2006 | Volume: 2 | Issue: 4 | Page No.: 426-430
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Research Article

Liver Regenerative Effect of Phyllanthus amarus Linn. Against Alcohol Induced Liver Cell Injury in Partially Hepatectomised Albino Rats

P. Chattopadhyay, S.S. Agrawal and A. Garg

ABSTRACT

The present study investigates the liver regenerative effect of Phyllathus amarus Linn against alcohol induced liver cell injury in partial hepatectomised albino rats. The oral administration of Phyllanthus amarus extracts increases the activities of thymidine kinase in regenerating rat liver at 24 h. Levels of DNA and protein analysis showed that the increase in thymidine kinase was caused by comparable increase the DNA and protein. Histopathology confirmed the mitosis counts increases to the activity of DNA. These findings suggested that Phyllanthus amarus inducing DNA synthesis by the induction of levels of synthesizing nucleic acid enzymes during liver regeneration. Phyllanthus amarus may be used, as a potential liver regenerative herb in hepatic disorders and seems to be beneficial against alcohol induced liver cell damage.
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REFERENCES

  1. Agrawal, S.S., A. Garg and S. Agarwal, 1986. Screening of Phyllanthus and Ricinus communis Linn on alcohol induced liver cell damage in non-hepatectomised and partially hepatectomised rats. Indian. J. Pharmacol., 18: 211-214.
    Direct Link
  2. Burton, K., 1956. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J., 62: 315-323.
    Direct Link
  3. Calixto, J.B., A.R.S. Santos, V.C. Filho and R.A. Yunes, 1998. A review of the plants of the genus Phyllanthus: Their chemistry, pharmacology and therapeutic potential. Med. Res. Rev., 18: 225-258.
    Direct Link
  4. Kassuya, C.A.L., F.P.L. Daniela, V.M. Lucilia, G.R. Vera-Lucia and B.C. Joao, 2005. Anti-inflammatory properties of extract, fractions and lignans isolated from Phyllanthus amarus. Planta Med., 71: 721-726.
    Direct Link
  5. Higgins, G.M. and R.M. Anderson, 1981. Experimental pathology of liver, Resortatio of liver of the white rat following partial surgical removal. Arch. Pathol., 186: 2220-2231.
  6. Jayaram, S., S.P. Thyagarajan, S. Sumathi, S. Manjula, S. Malathi and N. Madangopalan, 1997. Efficiency of Phyllanthus amarus treatment in acute viral hepatitis A, B and non A and Non B: An open clinical trial. Indian J. Virol., 13: 59-64.
  7. Joy, K.L. and R. Kuttan, 1998. Inhibitation by Phyllanthus amarus of hepatocarcinogenesis induced by N-Nitrosodiethylamine. J. Clin. Biochem. Nutr., 24: 133-139.
    Direct Link
  8. Kassuya, C.A.L., A.A. Silverstre, V. Rehder and J.B. Calixto, 2003. Anti-allodynic and anti-oedematogenic properties of the lignan from Phyllanthus amarus in models of persistant inflammatory and neuropathic pain. Eur. J. Pharmacol., 478: 145-153.
    Direct Link
  9. Kiemer, A.K., T. Hartung, C. Huber and A.M. Vollmer, 2003. Phyllanthus amarus has anti-inflammatory potential by inos, COX-2 and cytokines via the NF-kappa B Pathway. J. Hepatol., 38: 289-297.
    CrossRefDirect Link
  10. Lee, C.D., M. Ott, S.P. Thyagarajan, D.A. Shafritz, R.D. Burk and S. Gupta, 1996. Phyllanthus amarus down‐regulates hepatitis B virus mRNA transcription and replication. Eur. J. Clin. Invest., 26: 1069-1076.
    CrossRefDirect Link
  11. Levy, C.M. and T. Chen, 1979. Ethanol inhibition of liver regeneration. Gasteroentrology, 77: 1151-1153.
  12. Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall, 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem., 193: 265-275.
    CrossRefPubMedDirect Link
  13. Morley, C.G.D. and V.L. Royse, 1981. Adenergic agents as possible regulator of liver regeneration. Int. J. Biochem., 13: 969-973.
    PubMedDirect Link
  14. Nakata, R., I. Tsukamto, M. Miyoshi and S. Kojo, 1985. Liver regeneration after Carbontetrachloride introduction in the rat. Biochem. Pharmacol., 39: 586-587.
  15. Nakata, R., I. Tsukamto, M. Nanme, S. Mikino, M. Miyoshi and S. Kojo, 1985. α Adenergic regulation of the activity of thymidylate synthetase and thymidine kinase during liver regeneration after partial hepatectomy. Eur. J. Pharmacol., 114: 355-360.
    CrossRef
  16. Nakata, R., I. Tsukamto, M. Miyoshi and S. Kojo, 1987. Effect of thyroparathyoidectomy on the activities of thymidylate synthetase and thymidine kinase during liver regeneration after partial hepatectomy. Clin. Sci., 72: 455-457.
  17. Khan, D., J. Stadler, J. Twerbalache and R. van Hoorn-Hickman, 1980. Thymidine kinase: An expensive index of liver regeneration in a large animal model. Gastroenterology, 79: 907-911.
    PubMedDirect Link
  18. Ott, M., S.P. Thyagarajan and S. Gupta, 1997. Phyllanthus amrus suppress hepatitis virus by interrupting interaction between HBV enhancer I and cellular transcription factors. Eur. J. Clin. Invest., 27: 908-915.
  19. Rajeshkumar, N.V. and R. Kuttan, 2000. Phyllanthus amarus extract administration increases the life span of rats with hepatocellular carcinoma. J. Ethnopharmacol., 73: 215-219.
  20. Raphel, K.R. and R. Kuttan, 2003. Inhibition of experimental gastric lesions and inflammation by Phyllanthus amarus extract. J. Ethnopharmacol., 87: 193-197.
    CrossRefDirect Link
  21. Tzirogiannis, K.N., G.I. Panoutsopoulos, M.D. Demmonakou, R.I. Hereti, K.N. Alexandropoulou, A.C. Basayannis and M.G. Mykoniatis, 2003. Time-course of cadmium-induced acute hepatotoxicity in the rat liver: The role of apoptosis. Arch. Toxicol., 77: 694-701.
    CrossRefDirect Link
  22. Venkatswaran, P.S., I. Milliman and B.S. Blumberg, 1987. Effects of extract from Phyllanthus niruri on hepatitis B and woodchuck hepatitis viruses: In vitro and <in vivo studies. Proc. Natl. Acad. Sci. USA., 84: 274-278.
    PubMedDirect Link
  23. Wands, J.R., E.A. Carter, N.L. Bucher and K.J. Isebacher, 1979. Inhibition of hepatic regeneration in rats acute and chronic by ethanol intoxication. Gasteroentrology, 77: 528-531.
    PubMedDirect Link
  24. Thyagarajan, S.P., T. Thirunalasundari, S. Subramanian, P.S. Venkateswaran and B.S. Blumberg, 1988. Effect of Phyllanthus amarus on chronic carriers of hepatitis B virus. Lancet, 332: 764-776.
    CrossRefDirect Link

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Abraham olapade Reply

Can phyllanthus amarus cure hepatits b?

Editor

There is some scientific evidence to suggest that Phyllanthus amarus (also known as "bhumi amla" or "stonebreaker") may have some beneficial effects for people with hepatitis B, but it is not a cure for the virus.

Some studies have shown that Phyllanthus amarus may help reduce liver damage and inflammation associated with hepatitis B infection, as well as improve liver function. However, more research is needed to confirm these findings and determine the optimal dosages and treatment regimens.

It's important to note that hepatitis B is a serious viral infection that can lead to liver damage, cirrhosis, and liver cancer if left untreated. While some herbal remedies may have some potential benefits, they should never be used as a substitute for medical treatment or advice from a healthcare professional. If you have hepatitis B, it's important to work closely with your healthcare provider to manage your condition and receive appropriate medical care.

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