Abstract: The aim of this study was to investigate the comparative
effects of aminoglycosides and fluoroquinolones on testis apoptosis and
sperm parameters in rats. Fifty male Wistar rats were randomly divided
into control (n = 10) and experimental (n = 40) groups. The experimental
groups subdivided into four groups of ten. Each received 5 mg kg-1
(IP) gentamicin, 50 mg kg-1 (IP) neomycin, 40 mg kg-1
(IP) streptomycin and 72 mg kg-1 (IP) ofloxacin daily for 14
days, respectively; however, the control group just received vehicle (IP).
In the fourteenth day, rats were killed and sperm analyzed for sperm parameters.
Testis tissues were also prepared for TUNEL assay for detection of apoptosis.
There was a significant decrease in sperm count, viability and motility
in all of experimental groups when compared with control group. Although
in streptomycin group these parameters were less decreased than in the
other experimental groups. The apoptotic cells were significantly increased
in all experimental groups when compared with those seen in the controlled
group. Gentamicin, neomycin and streptomycin and ofloxacin have negative
effects on sperm parameters and testis apoptosis in rats. However, these
side effects are less seen in the streptomycin group. Therefore, it is
recommended that usage of this drug have fewer side effects on male fertility.